Estradiol, but not dehydroepiandrosterone, decreases parasitemia and increases the incidence of cerebral malaria and the mortality in Plasmodium berghei ANKA-infected CBA mice

xmlui.dri2xhtml.METS-1.0.item-date
2006xmlui.mirage2.itemSummaryView.MetaData
xmlui.ArtifactBrowser.ItemViewer.show_fullxmlui.dri2xhtml.METS-1.0.item-uri
http://patua.iec.gov.br/handle/iec/1053xmlui.dri2xhtml.METS-1.0.item-author
Libonati, Rosana Maria Feio
Cunha, Maristela G
Souza, José Maria de
Santos, Marcos V. N
Oliveira, Salma Gomes de
Daniel-Ribeiro, Claudio T
Carvalho, Leonardo José de Moura
Nascimento, José L. M. do
xmlui.dri2xhtml.METS-1.0.item-abstract
Objective: The effect of castration and subsequent replacement of dehydroepiandrosterone (DHEA) or estradiol
on parasitemia, mortality and incidence of cerebral
malaria (CM) was evaluated in CBA mice infected with
Plasmodium berghei ANKA. Methods: Female mice
were castrated, and groups of 12–15 animals received
daily injections of DHEA, estradiol or saline. Four days
after the start of treatment, mice were inoculated
with 1 ! 106 P. berghei ANKA-parasitized erythrocytes.
DHEA treatment was continued during the 5 days after
infection, and estradiol was administered during the follow-up.
Parasitemia was evaluated daily in Giemsastained
blood smears. Signs of CM were determined by
the manifestation of coma, limb paralysis and/or convulsions.
Plasma TNF- levels were evaluated by sandwich
ELISA. Nitric oxide synthase (NOS) activity in the brain
of moribund mice was measured by the method of Bredt
and Snyder. Results: In non-castrated infected mice, the
incidence of CM was 50%, and plasma TNF- increased
and brain NOS activity decreased compared to non-infected
controls. Castration had no major effect on the
parameters analyzed (parasitemia, mortality, CM incidence, TNF- levels or NOS activity). Estradiol replacement caused a decrease in parasitemia but resulted in
higher CM incidence and faster mortality, with an increase
in NOS activity. Conclusions: Estradiol modulated
the immune response of P. berghei ANKA-infected CBA
mice, decreasing parasitemia and increasing NOS activity,
and impacted negatively on survival and CM incidence,
showing that neuroimmunoendocrine interactions
are important in the physiopathogenesis of
malaria infections.
xmlui.dri2xhtml.METS-1.0.item-citation
LIBONATI, Rosana Maria Feio et al. Estradiol, but not dehydroepiandrosterone, decreases parasitemia and increases the incidence of cerebral malaria and the mortality in Plasmodium berghei ANKA-infected CBA mice. NeuroImmunoModulation, v. 13, n. 1, p. 28-35, 2006xmlui.dri2xhtml.METS-1.0.item-decsPrimary
Estradiol / administração & dosagemEstradiol / uso diagnóstico
Parasitemia / parasitologia
Plasmodium berghei / parasitologia
Malária Cerebral / mortalidade
Malária Cerebral / parasitologia
Camundongos Endogâmicos CBA / parasitologia
Castração