First detection of a human astrovirus type 8 in a child with diarrhea in Belém, Brazil: comparison with other strains worldwide and identification of possible three lineages
Gabbay, Yvone Benchimol
Linhares, Alexandre da Costa
Oliveira, Darleise de Souza
Nakamunra, Lilian S
Mascarenhas, Joana D'Arc Pereira
Gusmão, Rosa Helena P
Heinemann, Marcos B
Leite, José Paulo G
This study describes the genetic relationships of the first human astrovirus type-8 (HAstV-8) detected in Belém-Brazil, during a public hospital-based study. This strain was compared with other HAstV-8 strains identified elsewhere which have sequences available at GeneBank. The regions ORF1a (primers Mon348/Mon340) and ORF2 (primers Mon269/Mon270) were analyzed by nucleotide sequencing and a high similarity rate was observed among the Belém strain and other HAstV-8 strains. In ORF1a, homology values of 93-100% were detected, and in ORF2 96-99%. Considering the sequence variation (7%) observed in ORF2 region, it was suggested that HAstV-8 strains could be divided in three different lineages.
ReferenciaGABBAY, Yvone Benchimol et al. First detection of a human astrovirus type 8 in a child with diarrhea in Belém, Brazil: comparison with other strains worldwide and identification of possible three lineages. Memórias do Instituto Oswaldo Cruz, v. 102, n. 4, p. 531-534, June 2007.
DeCsMamastrovirus / isolamento & purificação
Diarréia Infantil / virologia
Mamastrovirus / classificação
Astrovirus type 8