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dc.contributor.authorReady, Paul D-
dc.contributor.authorLainson, Ralph-
dc.contributor.authorShaw, Jeffrey Jon-
dc.contributor.authorSouza, Adelson Alcimar Almeida de-
dc.date.accessioned2017-06-05T17:44:45Z-
dc.date.available2017-06-05T17:44:45Z-
dc.date.issued1991-
dc.identifier.citationREADY, Paul D. et al. DNA probes for distinguishing Psychodopygus wellcomei from Psychodopygus complexus (Diptera: Psychodidae). Memórias do Instituto Oswaldo Cruz, v. 86, n. 1, p. 41-49, jan.- maio 1991.pt_BR
dc.identifier.issn0074-0276-
dc.identifier.urihttp://patua.iec.gov.br/handle/iec/2580-
dc.description.abstractGenomic DNA fragments from males of Psychodopygus wellcomei were isolated and shown to be useful as sensitive diagnostic probes for positively separating individuais of this species from those of Ps. complexus. These two members of the Ps. squamiventris series are found sympatrically in foci of cutaneous leishmaniasis in the hill forests of southern Pará State. Of the two species, only Ps. wellcomei is thought to be an important vector of Leishmania braziliensis sensu stricto, but this is based on circumstantial evidence because of the difficulties of identifying female sandflies within the series. The diagnostic probes were isolated from a library ofPs. wellcomei built by ligating short fragments of Sau 3A-restricted, genomic DNA into the plasmid vector pUC 18. Differential screening of 1316 library clones with total genomic DNA of Ps. wellcomei and Ps. complexus identified 5 recombinants, with cross-hybridizing inserts of repetitive DNA, that showed strong specificity for Ps. wellcomei. As little as 0.4% of the DNA extracted from an individual sandfly (= ca. 0.5 nanogramsj was specifically detected. The diagnostic probes were used to identify as Ps. wellcomei a wild-caught female sandfly found infected with L. braziliensis s. s., providing only the second positive association between these two speciespt_BR
dc.language.isoengpt_BR
dc.publisherInstituto Oswaldo Cruzpt_BR
dc.rightsAcesso Abertopt_BR
dc.titleDNA probes for distinguishing Psychodopygus wellcomei from Psychodopygus complexus (Diptera: Psychodidae)pt_BR
dc.typeArtigopt_BR
dc.subject.decsPrimaryLeishmaniosept_BR
dc.subject.decsPrimaryDNApt_BR
dc.subject.decsPrimaryHibridização de Ácido Nucleicopt_BR
dc.subject.decsPrimaryCulicidaept_BR
dc.subject.decsPrimaryPsychodopygus wellcomeipt_BR
dc.creator.affilliationNatural History Museum. Entomology Department. Cromwell Road, London, U.K.pt_BR
dc.creator.affilliationMinistério da Saúde. Fundação Nacional de Saúde. Instituto Evandro Chagas. Belém, PA, Brasil.pt_BR
dc.creator.affilliationMinistério da Saúde. Fundação Nacional de Saúde. Instituto Evandro Chagas. Belém, PA, Brasil.pt_BR
dc.creator.affilliationMinistério da Saúde. Fundação Nacional de Saúde. Instituto Evandro Chagas. Belém, PA, Brasil.pt_BR


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