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dc.contributor.authorCooke, S. J-
dc.contributor.authorClarke, I. N-
dc.contributor.authorFreitas, Ronaldo Barros de-
dc.contributor.authorGabbay, Yvone Benchimol-
dc.contributor.authorLambden, P. R-
dc.date.accessioned2017-06-09T19:14:04Z-
dc.date.available2017-06-09T19:14:04Z-
dc.date.issued1992-
dc.identifier.citationCOOKE, S. J. et al. The correct sequence of the porcine group C/Ccowden rotavirus major inner capsid protein shows close homology with human isolates from Brazil and the U.K. Virology, v. 190, n. 1, p. 531-537, 1992.pt_BR
dc.identifier.issn0042-6822-
dc.identifier.urihttp://patua.iec.gov.br/handle/iec/2602-
dc.description.abstractAmino acid sequence alignments between the human group C/Bristol and the published porcine group C/Cowden VP6 proteins have revealed a region of extreme sequence divergence. We have been unable to confirm the nucleotide sequence of the Cowden VP6 gene corresponding to this region of divergence. Direct sequencing of a PCR-amplified cDNA pool has revealed a frame shift, and three nucleotide changes, within the published sequence of the porcine (Cowden) VP6 gene. The corrected sequence of the porcine protein revealed a closer homology with VP6 from the Bristol strain and two new human group C rotavirus isolates. Atypical rotaviruses have been detected in the feces of children living in Belem, Brazil, and Preston, U.K. Direct sequencing of PCR-amplified cDNA corresponding to the VP6 gene of one isolate from each location confirmed the presence of a group C rotavirus. The complete nucleotide sequences of the VP6 genes from the group C/Belem and C/Preston rotaviruses contained an open reading frame of 1185 nucleotides (395 amino acids; deduced Ivl, 44,669 Da). The Belem VP6 gene demonstrated 97.9% nucleotide homology with the human group C/Bristol VP6 gene and 83.4% nucleotide homology (91.6% deduced amino acid homology) with the corrected porcine group C/Cowden sequence. The Preston VP6 gene demonstrated 99.6% nucleotide homology with the human group C/Bristol VP6 gene and 84.0% nucleotide homology (91.6% deduced amino acid homology) with the corrected porcine group C/Cowden sequence. Remarkably, the deduced amino acid sequence of the Brazilian strain was identical to that of the U.K. isolates.pt_BR
dc.language.isoengpt_BR
dc.publisherElsevierpt_BR
dc.rightsAcesso Abertopt_BR
dc.titleThe correct sequence of the porcine group C/Ccowden rotavirus major inner capsid protein shows close homology with human isolates from Brazil and the U.K.pt_BR
dc.typeArtigopt_BR
dc.subject.decsPrimaryRotaviruspt_BR
dc.subject.decsPrimaryInfecções por Rotaviruspt_BR
dc.subject.decsPrimaryGastroenteritept_BR
dc.subject.decsPrimaryProteínas de Ligação a DNApt_BR
dc.subject.decsPrimaryDNA / análisept_BR
dc.creator.affilliationUniversity Medical School. Southampton General Hospital. Department of Microbiology. Tremona Road, Southampton, U.K.pt_BR
dc.creator.affilliationUniversity Medical School. Southampton General Hospital. Department of Microbiology. Tremona Road, Southampton, U.K.pt_BR
dc.creator.affilliationMinistério da Saúde. Fundação Nacional de Saúde. Instituto Evandro Chagas. Belém, PA, Brasil.pt_BR
dc.creator.affilliationMinistério da Saúde. Fundação Nacional de Saúde. Instituto Evandro Chagas. Belém, PA, Brasil.pt_BR
dc.creator.affilliationUniversity Medical School. Southampton General Hospital. Department of Microbiology. Tremona Road, Southampton, U.K.pt_BR


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