Analysis of constitutive heterochromatin of Aotus (Cebidae, Primates) by restriction enzyme and fluorochrome bands

xmlui.dri2xhtml.METS-1.0.item-date
1998xmlui.dri2xhtml.METS-1.0.item-files-viewOpen
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http://patua.iec.gov.br//handle/iec/2721xmlui.dri2xhtml.METS-1.0.item-author
Pieczarka, J. C
Nagamachi, C. Y
Muniz, José Augusto Pereira Carneiro
Barros, R. M. S
Mattev, M. S
xmlui.dri2xhtml.METS-1.0.item-abstract
The current classication of genus Aotus includes
nine species, four of which occur above the Amazon
River and five below it. The position of several of
these taxa as a valid species has been questioned.
Recently, we described the chromosomal constitution
of a population in the state of Rondonia, Brazil,
whose karyotype typically presented a considerable
accumulation of constitutive heterochromatin. To
best characterize these heterochromatins, in this
work we subjected the metaphases of these animals
to banding using AluI, HaeIII, HinfI, RsaI, DdeI, MboI
and MspI restriction enzymes and CMAᶾ and DAPI
fluorochromes. The banded metaphases were also
submitted to sequential C-banding. RsaI, DdeI and
MboI enzymes showed, in all chromosomes, a banding
pattern of C type, similar to that obtained using
barium hydroxide. This banding was also seen with
AluI, Hinf I and MspI, but with reduction or elimination
of the C-bands in the chromosome pairs 1, 3-7
and 9. MspI also reduced the C-band of pairs 11, 16-
21 and 23. HaeIII induced intermediate bands between
G and C. Considering the data of the different
bands produced, it was possible to characterize at
least three distinct types of constitutive heterochromatin
in Aotus from Rondonia: (a) centromeric
bands, (b) bands of the heterochromatic short arms
and (c) interstitial bands.
xmlui.dri2xhtml.METS-1.0.item-citation
PIECZARKA, J. C. et al. Analysis of constitutive heterochromatin of Aotus (Cebidae, Primates) by restriction enzyme and fluorochrome bands. Chromosome Research, v. 6, n. 2, p. 77-83, Feb. 1998xmlui.dri2xhtml.METS-1.0.item-decsPrimary
Aotus trivirgatus / genéticaBandeamento Cromossômico / métodos
Corantes Fluorescentes
Heterocromatina / genética