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dc.contributor.authorNunes, Marcio Roberto Teixeira-
dc.contributor.authorVianez Júnior, João Lídio da Silva Gonçalves-
dc.contributor.authorNunes, Keley Nascimento Barbosa-
dc.contributor.authorSilva, Sandro Patroca da-
dc.contributor.authorLima, Clayton Pereira Silva de-
dc.contributor.authorGuzman, Hilda-
dc.contributor.authorMartins, Lívia Caricio-
dc.contributor.authorCarvalho, Valéria Lima-
dc.contributor.authorTesh, Robert B-
dc.contributor.authorVasconcelos, Pedro Fernando da Costa-
dc.date.accessioned2017-09-22T09:47:38Z-
dc.date.available2017-09-22T09:47:38Z-
dc.date.issued2015-
dc.identifier.citationNUNES, Marcio Roberto Teixeira et al. Analysis of a reverse transcription loop-mediated isothermal amplification (RT-LAMP) for yellow fever diagnostic. Journal of Virological Methods, v. 226, p. 40-51, Dec. 2015.pt_BR
dc.identifier.issn1879-0984-
dc.identifier.urihttp://patua.iec.gov.br//handle/iec/2760-
dc.description.abstractYellow Fever virus (YFV) is an important human pathogen in tropical areas of Africa and South America. Although an efficient vaccine is available and has been used since the early 1940s, sylvatic YFV transmission still occurs in forested areas where anthropogenic actions are present, such as mineral extraction, rearing livestock and agriculture, and ecological tourism. In this context, two distinct techniques based on the RT-PCR derived method have been previously developed, however both methods are expensive due to the use of thermo cyclers and labeled probes. We developed isothermal genome amplification, which is a rapid, sensitive, specific and low cost molecular approach for YFV genome detection. This assay used a set of degenerate primers designed for the NS1 gene and was able to amplify, within 30 min in isothermal conditions, the YFV 17D vaccine strain derived from an African wild prototype strain (Asibi),as well as field strains from Brazil, other endemic countries from South and Central America, and the Caribbean. The generic RT-LAMP assay could be helpful for YFV surveillance in field and rapid response during outbreaks in endemic areas.pt_BR
dc.description.sponsorshipThis study was partially supported by CNPq (Conselho Nacional para o Desenvolvimento Científico e Tecnológico) projects 401558/2013-4; 457664/2013-4, 486069/2012-5 and 301641/2010-2), and CNPq/CAPES/FAPESPA (project 573739-2008-0).pt_BR
dc.language.isoengpt_BR
dc.publisherElsevierpt_BR
dc.rightsAcesso Abertopt_BR
dc.titleAnalysis of a reverse transcription loop-mediated isothermal amplification (RT-LAMP) for yellow fever diagnosticpt_BR
dc.typeArtigopt_BR
dc.subject.decsPrimaryVírus da Febre Amarela / isolamento & purificaçãopt_BR
dc.subject.decsPrimaryFebre Amarela / diagnósticopt_BR
dc.subject.decsPrimaryFebre Amarela / virologiapt_BR
dc.subject.decsPrimaryTranscrição Reversapt_BR
dc.subject.decsPrimaryReação em Cadeia da Polimerase Via Transcriptase Reversa / métodospt_BR
dc.subject.decsPrimaryTécnicas de Amplificação de Ácido Nucleico / métodospt_BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Centro de Inovações Tecnológicas. Ananindeua, PA, Brasil.pt_BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Centro de Inovações Tecnológicas. Ananindeua, PA, Brasil.pt_BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Centro de Inovações Tecnológicas. Ananindeua, PA, Brasil.pt_BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Centro de Inovações Tecnológicas. Ananindeua, PA, Brasil.pt_BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Centro de Inovações Tecnológicas. Ananindeua, PA, Brasil.pt_BR
dc.creator.affilliationUniversity of Texas Medical Branch. Department of Pathology. Galveston, TX, USA.en_US
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.pt_BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.pt_BR
dc.creator.affilliationUniversity of Texas Medical Branch. Department of Pathology. Galveston, TX, USA.en_US
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil / University of Para State. Department of Pathology. Belem, PA, Brazil.pt_BR
dc.identifier.doi10.1016/j.jviromet.2015.10.003-


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