Rotavirus infection in wild marsupials (Didelphis marsupialis) of the Amazon region
Linhares, Alexandre da Costa
Pereira, J. D. M
Nakauth, C. M
Gabbay, Yvone Benchimol
Rotavirus was detected by enzyme-linked immunosorbent assay in faecal specimens collected from two (1.35%) of 148 marsupials trapped in the Amazon jungle environment. The positive samples were both from the "common opossum", Didelphis marsupialis. No infections were found in the stools of 198 animals belonging to other mammalian species: the latter included small rodents, chiropterans and primates. Electron microscopic examination of one (MA 5928) rotavirus-positive specimen showed a large number of empty particles. However, both rotavirus strains grew when inoculated in MA 104 cells (foetal Rhesus monkey kidney cells) producing clear cytopathogenic effect; indirect immunofluorescence technique of these cells showed a typical granular cytoplasmic fluorescence. The electrophoretic profile of strain MA 5928 showed a high grade of homology with that of SA 11, but also showed minor differences.
xmlui.dri2xhtml.METS-1.0.item-citationLINHARES, Alexandre da Costa et al. Rotavirus infection in wild marsupials (Didelphis marsupialis) of the Amazon region. Transactions of The Royal Society of Tropical Medicine and Hygiene, v. 80, n. 1, p. 20-24, 1986.
Infecções por Rotavirus / epidemiologia
Rotavirus / isolamento & purificação
Infecções por Rotavirus / transmissão
Infecções por Rotavirus
Ensaio de Imunoadsorção Enzimática / métodos