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dc.contributor.authorMiles, M. A-
dc.contributor.authorB, W. Apt-
dc.contributor.authorWidmer, G-
dc.contributor.authorPóvoa, Marinete Marins-
dc.contributor.authorSchofield, C. J-
dc.date.accessioned2017-09-26T14:07:24Z-
dc.date.available2017-09-26T14:07:24Z-
dc.date.issued1984-
dc.identifier.citationMILES, M. A. et al. Isozyme heterogeneity and numerical taxonomy of Trypanosoma cruzi stocks from Chile. Transactions of The Royal Society of Tropical Medicine and Hygiene, v. 78, n. 4, p. 526-535, 1984.pt_BR
dc.identifier.issn0035-9203-
dc.identifier.urihttp://patua.iec.gov.br//handle/iec/2765-
dc.description.abstractFifty-three stocks of Trypanosoma cruzi were isolated in Chile, 13 from patients, 32 from the domestic triatomine vector Triatoma infestans, and 8 from the silvatic and peridomestic vector T. spinolai. The majority of isolates from triatomine bugs were made by the direct culture of infected faeces. The 53 stocks and a single clone were characterized by a combination of starch-gel and cellulose acetate enzyme electrophoresis. Three groups of T. cruzi stocks were apparent from either simple visual comparisons of isozyme profiles or numerical taxonomy. The groups were designated Chilean zymodeme (Z) 1, which was similar to Brazilian Z1, Chilean Z2a, similar to Brazilian Z2 and Chilean Z2b, similar to Bolivian Z2 and with prominent heterozygous isozyme profiles. Chilean Z1 was isolated only from T. spinolai colonizing farm walls inhabited by the rodent Octodon degus. Chilean Z2a and Z2b were both isolated from domestic T. infestans T. infestans and man, in some cases within the same household. Hardy-Weinberg equilibria were not found amongst a group of 22 stocks from a single locality and deviations from theoretical Hardy-Weinberg distributions were compatible with the absence of genetic exchange in the sampled population of T. cruzi.pt_BR
dc.language.isoengpt_BR
dc.publisherOxford University Presspt_BR
dc.rightsAcesso Abertopt_BR
dc.titleIsozyme heterogeneity and numerical taxonomy of Trypanosoma cruzi stocks from Chilept_BR
dc.typeArtigopt_BR
dc.subject.decsPrimaryTrypanosoma cruzipt_BR
dc.subject.decsPrimaryTrypanosoma cruzi / enzimologiapt_BR
dc.subject.decsPrimaryTrypanosoma cruzi / genéticapt_BR
dc.subject.decsPrimaryTrypanosoma cruzi / classificaçãopt_BR
dc.subject.decsPrimaryEletroforese em Acetato de Celulose / métodospt_BR
dc.subject.decsPrimaryEletroforese em Gel de Amido / métodospt_BR
dc.subject.decsPrimaryIsoenzimas / análisept_BR
dc.subject.decsPrimaryAmérica do Sulpt_BR
dc.subject.decsPrimaryChile (CL)pt_BR
dc.creator.affilliationMinistério da Saúde. Fundação Serviços de Saúde Pública. Instituto Evandro Chagas. Belém, PA, Brasil / London School of Hygiene and Tropical Medicine. Departament of Medical Protozoologv and Departament of Medical Entomology. Keppel Street, London.pt_BR
dc.creator.affilliationUniversity of Chile. Faculty of Medicine. Department of Experimental Medicine. Parasitology Unit. Santiago, Chile.pt_BR
dc.creator.affilliationMinistério da Saúde. Fundação Serviços de Saúde Pública. Instituto Evandro Chagas. Belém, PA, Brasil.pt_BR
dc.creator.affilliationLondon School of Hygiene and Tropical Medicine. Departament of Medical Protozoologv and Departament of Medical Entomology. Keppel Street, London.pt_BR
dc.identifier.doi10.1016/0035-9203(84)90076-2-


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