Detection of the VP6 gene of group F and G rotaviruses in broiler chicken fecal samples from the Amazon region of Brazil
Mascarenhas, Joana D'Arc Pereira
Bezerra, Delana A. M
Silva, René R
Silva, Mayara J. M
Sousa Junior, Edivaldo Costa
Soares, Luana Souza
The aim of this study was to detect rotavirus F (RVF) and rotavirus G (RVG) in fecal specimens of broiler chickens in Brazil. During 2008 and 2011, a total of 85 fecal samples were collected. The viral genome was extracted, followed by polyacrylamide gel electrophoresis (PAGE), reverse transcription polymerase chain reaction (RT-PCR), and nucleotide sequencing. Samples were screened for rotaviruses by PAGE, and RVF and RVG genome banding patterns were not seen. Using RT-PCR, it was found that 9.4 % (8/85) of the pools contained RVF, whereas 10.6 % (9/85) contained RVG. The predicted amino acid sequences of RVF and RVG from Brazilian samples were 94.4-95.7 % and 96.8-96.9 % identical, respectively, to those of prototypes from Germany. The detection of RVF and RVG in this study provides important epidemiological data about the simultaneous circulation of rotaviruses affecting broiler flocks in the Amazon region of Brazil.
xmlui.dri2xhtml.METS-1.0.item-citationMASCARENHAS, Joana D’arc Pereira et al. Detection of the VP6 gene of group F and G rotaviruses in broiler chicken fecal samples from the Amazon region of Brazil. Archives of Virology, v. 161, n. 8, p. 2263-2268, Aug. 2016.
Infecções por Rotavirus / epidemiologia
Galinhas / virologia
Fezes / virologia
Reação em Cadeia da Polimerase Via Transcriptase Reversa / métodos
Eletroforese em Gel de Poliacrilamida / métodos