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dc.contributor.authorMascarenhas, Joana D'Arc Pereira-
dc.contributor.authorBezerra, Delana A. M-
dc.contributor.authorSilva, René R-
dc.contributor.authorSilva, Mayara J. M-
dc.contributor.authorSousa Junior, Edivaldo Costa-
dc.contributor.authorSoares, Luana Souza-
dc.date.accessioned2017-11-03T18:05:48Z-
dc.date.available2017-11-03T18:05:48Z-
dc.date.issued2016-
dc.identifier.citationMASCARENHAS, Joana D’arc Pereira et al. Detection of the VP6 gene of group F and G rotaviruses in broiler chicken fecal samples from the Amazon region of Brazil. Archives of Virology, v. 161, n. 8, p. 2263-2268, Aug. 2016.pt_BR
dc.identifier.issn1432-8798-
dc.identifier.urihttp://patua.iec.gov.br//handle/iec/2822-
dc.description.abstractThe aim of this study was to detect rotavirus F (RVF) and rotavirus G (RVG) in fecal specimens of broiler chickens in Brazil. During 2008 and 2011, a total of 85 fecal samples were collected. The viral genome was extracted, followed by polyacrylamide gel electrophoresis (PAGE), reverse transcription polymerase chain reaction (RT-PCR), and nucleotide sequencing. Samples were screened for rotaviruses by PAGE, and RVF and RVG genome banding patterns were not seen. Using RT-PCR, it was found that 9.4 % (8/85) of the pools contained RVF, whereas 10.6 % (9/85) contained RVG. The predicted amino acid sequences of RVF and RVG from Brazilian samples were 94.4-95.7 % and 96.8-96.9 % identical, respectively, to those of prototypes from Germany. The detection of RVF and RVG in this study provides important epidemiological data about the simultaneous circulation of rotaviruses affecting broiler flocks in the Amazon region of Brazil.pt_BR
dc.description.sponsorshipThis study was partially supported by a grant from Pará State Secretary of Science and Technology, contract no. 067/2008 (SEDECT/FAPESPA/PA).pt_BR
dc.language.isoengpt_BR
dc.publisherSpringer Verlagpt_BR
dc.rightsAcesso Embargadopt_BR
dc.titleDetection of the VP6 gene of group F and G rotaviruses in broiler chicken fecal samples from the Amazon region of Brazilpt_BR
dc.typeArtigopt_BR
dc.subject.decsPrimaryRotaviruspt_BR
dc.subject.decsPrimaryInfecções por Rotavirus / epidemiologiapt_BR
dc.subject.decsPrimaryAves Domésticaspt_BR
dc.subject.decsPrimaryGalinhas / virologiapt_BR
dc.subject.decsPrimaryFezes / virologiapt_BR
dc.subject.decsPrimaryEcossistema Amazônicopt_BR
dc.subject.decsPrimaryReação em Cadeia da Polimerase Via Transcriptase Reversa / métodospt_BR
dc.subject.decsPrimaryEletroforese em Gel de Poliacrilamida / métodospt_BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.pt_BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.pt_BR
dc.creator.affilliationMinistério da Agricultura. Laboratório Nacional Agropecuário. Belém, PA, Brazil.pt_BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.pt_BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.pt_BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.pt_BR
dc.identifier.doi10.1007/s00705-016-2882-z-


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