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dc.contributor.authorCabral-Castro, Mauro Jorge-
dc.contributor.authorPeralta, Regina Helena Saramago-
dc.contributor.authorCavalcanti, Marta Guimarães-
dc.contributor.authorPuccioni-Sohler, Marzia-
dc.contributor.authorCarvalho, Valéria Lima-
dc.contributor.authorVasconcelos, Pedro Fernando da Costa-
dc.contributor.authorPeralta, José Mauro-
dc.date.accessioned2017-11-06T16:34:28Z-
dc.date.available2017-11-06T16:34:28Z-
dc.date.issued2016-
dc.identifier.citationCABRAL-CASTRO, Mauro Jorge et al. A Luminex-based single DNA fragment amplification assay as a practical tool for detecting and serotyping dengue virus. Journal of Virological Methods, v. 236, p. 18-24, Oct. 2016.pt_BR
dc.identifier.issn0166-0934-
dc.identifier.urihttp://patua.iec.gov.br//handle/iec/2827-
dc.description.abstractDengue is a mosquito-borne viral infection that can evolve from subclinical to severe forms of disease. Early recognition during initial primary and secondary infections correlates with a reduced case-fatality rate in susceptible groups. The aim of this study was to standardize a DNA hybridization assay based on the Luminex technology for detecting and serotyping dengue virus (DENV). Reference DENVs representing the four different serotypes were used as controls to standardize the test. For validation, 16 DENV isolates obtained from a reference laboratory were analyzed in a double-blind manner to validate the test. Sixty blood samples from patients suspected of having dengue fever were used to evaluate the methodology after the validation step, and the results were compared with the reference semi-nested RT-PCR. Additionally, five human samples of each Zika and Chikungunya confirmed patients were used for specificity analysis. The Luminex-based assay correctly identified all 16 DENV isolates. In the evaluation step, the results of the RT-PCR/Luminex assay showed a concordance of 86.7% with those of the semi-nested RT-PCR. None of other virus infection samples was amplified. This is the first description of a hybridization assay that can discriminate the four DENV serotypes using probes against a single DENV sequence. The results indicated that the RT-PCR/Luminex DENV assay designed and evaluated in this study is a valuable additional tool for the early and rapid detection and serotyping of DENV, which could, in the future, be applied to new targets such as the Zika and Chikungunya viruses.pt_BR
dc.description.sponsorshipThis work was supported by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Brazil and the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Brazil and Fundação de Amparo a Pesquisa Carlos Chagas Filho, Rio de Janeiro, Brazil.pt_BR
dc.language.isoengpt_BR
dc.publisherElsevierpt_BR
dc.rightsAcesso Abertopt_BR
dc.titleA Luminex-based single DNA fragment amplification assay as a practical tool for detecting and serotyping dengue viruspt_BR
dc.typeArtigopt_BR
dc.subject.decsPrimaryDengue / diagnósticopt_BR
dc.subject.decsPrimaryDengue / virologiapt_BR
dc.subject.decsPrimaryDiagnóstico Precocept_BR
dc.subject.decsPrimarySensibilidade e Especificidadept_BR
dc.subject.decsPrimaryReação em Cadeia da Polimerase Via Transcriptase Reversa / métodospt_BR
dc.creator.affilliationUniversidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brazil.pt_BR
dc.creator.affilliationUniversidade Federal Fluminense. Faculdade de Medicina. Departamento de Patologia. Niterói, RJ, Brazil.pt_BR
dc.creator.affilliationUniversidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brazil / Universidade Federal do Rio de Janeiroe. Hospital Universitário Clementino Fraga Filho. Dpartamento de Doenças Infecciosas e Parasitárias. Rio de Janeiro, RJ, Brazil.pt_BR
dc.creator.affilliationUniversidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Laboratório de Líquido Cefalorraqueano. Rio de Janeiro, RJ, Brazil.pt_BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.pt_BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.pt_BR
dc.creator.affilliationUniversidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brazil.pt_BR
dc.identifier.doi10.1016/j.jviromet.2016.07.003-


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