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dc.contributor.authorMarques, Fernanda A
dc.contributor.authorSoares, Rodrigo P
dc.contributor.authorAlmeida, Gregório G
dc.contributor.authorSouza, Carolina C
dc.contributor.authorMelo, Maria N
dc.contributor.authorPinto, Sebastião A
dc.contributor.authorQuixabeira, Valeria B
dc.contributor.authorPereira, Ledice I
dc.contributor.authorDorta, Miriam L
dc.contributor.authorRibeiro-Dias, Fatima
dc.contributor.authorSilveira, Fernando Tobias
dc.contributor.authorSilva, Sydnei M
dc.contributor.authorGontijo, Celia M
dc.contributor.authorTafuri, Wagner L
dc.date.accessioned2017-11-14T11:46:59Z
dc.date.available2017-11-14T11:46:59Z
dc.date.issued2017
dc.identifier.citationMARQUES, Fernanda A. et al. Effectiveness of an immunohistochemical protocol for Leishmania detection in different clinical forms of American tegumentary leishmaniasis. Parasitology International, v. 66, n. 1, p. 884-888, 2017.pt_BR
dc.identifier.issn1383-5769
dc.identifier.urihttp://patua.iec.gov.br//handle/iec/2844
dc.description.abstractAmerican tegumentary leishmaniasis (ATL) is a neglected disease widely distributed in Latin America. In Brazil, it is caused by different Leishmania species belonging to the Subgenera Viannia and Leishmania. ATL diagnosis is routinely based on clinical, epidemiological, parasitological and immunological (delayed-type hypersensitivity skin test-DTH) evidences. The main objective of this work was to determine the efficacy of a previous immunohistochemical (IHC) method developed by our group. Seventy eight skin biopsies from patients with different ATL clinical forms and origins were evaluated. The method was previously standardized in ATL patients from the municipality of Caratinga, Minas Gerais, Brazil, all infected with Leishmania (V.) braziliensis. Here, it is evaluated in patients from the North, Southeast and Midwest regions of Brazil. Clinical, parasitological (biopsy PCR) and immunological (Montenegro skin test-MST) diagnosis were performed prior to IHC procedure. The IHC procedure detected 70.5% of the cases having a high agreement with MST diagnosis (kappa=0.84). A distinguished contribution of this work is that IHC succeed in diagnosing some negative DTH patients. Those were infected with Leishmania (L.) amazonensis, commonly causing the anergic form of the disease. In conclusion, IHC succeed in detecting ATL caused by different Leishmania species from various geographic regions and clinical status. Although it was not able to detect ATL in all patients, it was better than MST providing an additional tool for the diagnosis of ATL patients. There was no significant correlation between clinical forms and histological features including the presence of necrosisen_US
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.rightsAcesso Embargadopt_BR
dc.titleEffectiveness of an immunohistochemical protocol for Leishmania detection in different clinical forms of American tegumentary leishmaniasisen_US
dc.typeArticlept_BR
dc.subject.decsPrimaryLeishmania / imunologiapt_BR
dc.subject.decsPrimaryLeishmania / isolamento & purificaçãopt_BR
dc.subject.decsPrimaryLeishmania braziliensis / imunologiapt_BR
dc.subject.decsPrimaryLeishmania braziliensis / isolamento & purificaçãopt_BR
dc.subject.decsPrimaryLeishmaniose Cutânea / diagnósticopt_BR
dc.subject.decsPrimaryLeishmaniose Cutânea / epidemiologiapt_BR
dc.subject.decsPrimaryLeishmaniose Cutânea / parasitologiapt_BR
dc.subject.decsPrimaryPele / parasitologiapt_BR
dc.subject.decsPrimaryPele / patologiapt_BR
dc.subject.decsPrimaryImuno-Histoquímica / métodospt_BR
dc.subject.decsPrimaryBrasil / epidemiologiapt_BR
dc.subject.decsPrimaryEstados Unidosen_US
dc.creator.affilliationUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Patologia Geral. Belo Horizonte, MG, Brazil.pt_BR
dc.creator.affilliationFundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Belo Horizonte, MG, Brazil.pt_BR
dc.creator.affilliationUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Patologia Geral. Belo Horizonte, MG, Brazil.pt_BR
dc.creator.affilliationUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Patologia Geral. Belo Horizonte, MG, Brazil.pt_BR
dc.creator.affilliationUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brazil.pt_BR
dc.creator.affilliationUniversidade Federal de Goiás. Instituto de Patologia Tropical e Saúde Pública. Goiânia, GO, Brazil.pt_BR
dc.creator.affilliationUniversidade Federal de Goiás. Instituto de Patologia Tropical e Saúde Pública. Goiânia, GO, Brazil.pt_BR
dc.creator.affilliationUniversidade Federal de Goiás. Faculdade de Medicina. Instituto Goiano de Oncologia e Hematologia. Goiânia, GO, Brazil.pt_BR
dc.creator.affilliationUniversidade Federal de Goiás. Faculdade de Medicina. Instituto Goiano de Oncologia e Hematologia. Goiânia, GO, Brazil.pt_BR
dc.creator.affilliationUniversidade Federal de Goiás. Faculdade de Medicina. Instituto Goiano de Oncologia e Hematologia. Goiânia, GO, Brazil.pt_BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brazil.pt_BR
dc.creator.affilliationUniversidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Departamento de Imunologia, Microbiologia e Parasitologia. Uberlândia, MG, Brazil.pt_BR
dc.creator.affilliationFundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Belo Horizonte, MG, Brazil.pt_BR
dc.creator.affilliationUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Patologia Geral. Belo Horizonte, MG, Brazil.pt_BR
dc.identifier.doi10.1016/j.parint.2016.10.003


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