xmlui.ArtifactBrowser.ItemViewer.show_simple

dc.contributor.authorFarias, Luis Henrique Seabra-
dc.contributor.authorRodrigues, Ana Paula Drummond-
dc.contributor.authorCoêlho, E. C.-
dc.contributor.authorSantos, M. F.-
dc.contributor.authorSampaio, S. C.-
dc.contributor.authorSilva, E. O.-
dc.date.accessioned2017-11-17T17:37:31Z-
dc.date.available2017-11-17T17:37:31Z-
dc.date.issued2017-
dc.identifier.citationFARIAS, Luis Henrique Seabra et al. Crotoxin stimulates an M1 activation profile in murine macrophages during Leishmania amazonensis infection. Parasitology, v. 144,n. 11, p. 1458-1467, Sept. 2017.pt_BR
dc.identifier.issn0031-1820-
dc.identifier.urihttp://patua.iec.gov.br//handle/iec/2855-
dc.description.abstractAmerican tegumentary leishmaniasis is caused by different species of Leishmania. This protozoan employs several mechanisms to subvert the microbicidal activity of macrophages and, given the limited efficacy of current therapies, the development of alternative treatments is essential. Animal venoms are known to exhibit a variety of pharmacological activities, including antiparasitic effects. Crotoxin (CTX) is the main component of Crotalus durissus terrificus venom, and it has several biological effects. Nevertheless, there is no report of CTX activity during macrophage - Leishmania interactions. Thus, the main objective of this study was to evaluate whether CTX has a role in macrophage M1 polarization during Leishmania infection murine macrophages, Leishmania amazonensis promastigotes and L. amazonensis-infected macrophages were challenged with CTX. MTT [3-(4,5dimethylthiazol-2-yl)-2,5-diphenyl tetrasodium bromide] toxicity assays were performed on murine macrophages, and no damage was observed in these cells. Promastigotes, however, were affected by treatment with CTX (IC50 = 22·86 µg mL-1) as were intracellular amastigotes. Macrophages treated with CTX also demonstrated increased reactive oxygen species production. After they were infected with Leishmania, macrophages exhibited an increase in nitric oxide production that converged into an M1 activation profile, as suggested by their elevated production of the cytokines interleukin-6 and tumour necrosis factor-α and changes in their morphology. CTX was able to reverse the L. amazonensis-mediated inhibition of macrophage immune responses and is capable of polarizing macrophages to the M1 profile, which is associated with a better prognosis for cutaneous leishmaniasis treatment.pt_BR
dc.language.isoengpt_BR
dc.publisherCambridge University Presspt_BR
dc.rightsAcesso Embargadopt_BR
dc.titleCrotoxin stimulates an M1 activation profile in murine macrophages during Leishmania amazonensis infectionpt_BR
dc.typeArtigopt_BR
dc.subject.decsPrimaryLeishmania / efeitos de drogaspt_BR
dc.subject.decsPrimaryLeishmaniose Cutânea / farmacologiapt_BR
dc.subject.decsPrimaryCrotoxina / efeitos adversospt_BR
dc.subject.decsPrimaryCrotoxina / farmacologiapt_BR
dc.subject.decsPrimaryCrotoxina / toxicidadept_BR
dc.subject.decsPrimaryCrotoxina / uso terapêuticopt_BR
dc.subject.decsPrimaryMacrófagos Peritoneais / imunologiapt_BR
dc.subject.decsPrimaryMacrófagos Peritoneais / efeitos de drogaspt_BR
dc.subject.decsPrimaryÓxido Nítrico / metabolismopt_BR
dc.subject.decsPrimaryEspécies Reativas de Oxigênio / metabolismopt_BR
dc.subject.decsPrimaryAtivação de Macrófagos / efeitos de drogaspt_BR
dc.subject.decsPrimaryFator Estimulador de Colônias de Macrófagos / efeitos de drogaspt_BR
dc.subject.decsPrimaryCitometria de Fluxo / métodospt_BR
dc.subject.decsPrimaryCamundongos Endogâmicos BALB Cpt_BR
dc.creator.affilliationFederal University of Para. Institute of Biological Sciences. Laboratory of Parasitology and Laboratory of Structural Biology. Belém, PA, Brazil / National Institute of Science and Technology in Structural Biology and Bioimaging. Rio de Janeiro, RJ, Brazil.pt_BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil / National Institute of Science and Technology in Structural Biology and Bioimaging. Rio de Janeiro, RJ, Brazil.pt_BR
dc.creator.affilliationFederal University of Para. Institute of Biological Sciences. Laboratory of Parasitology and Laboratory of Structural Biology. Belém, PA, Brazil.pt_BR
dc.creator.affilliationUniversity of São Paulo. Department,Institute of Biomedical Sciences. Cell and Developmental Biology. São Paulo, SP, Brazil.pt_BR
dc.creator.affilliationButantan Institute. Laboratory of Pathophysiology. São Paulo, SP, Brazil / University of São Paulo. Institute of Biomedical Sciences. Department of Pharmacology. São Paulo, SP, Brazil.pt_BR
dc.creator.affilliationFederal University of Para. Institute of Biological Sciences. Laboratory of Parasitology and Laboratory of Structural Biology. Belém, PA, Brazil / National Institute of Science and Technology in Structural Biology and Bioimaging. Rio de Janeiro, RJ, Brazil.pt_BR
dc.identifier.doi10.1017/S0031182017000944-


xmlui.dri2xhtml.METS-1.0.item-files-head

Thumbnail

xmlui.ArtifactBrowser.ItemViewer.head_parent_collections

xmlui.ArtifactBrowser.ItemViewer.show_simple