Norovirus genogroups I and II in environmental water samples from Belém city, Northern Brazil

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2017xmlui.dri2xhtml.METS-1.0.item-files-viewOpen
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http://patua.iec.gov.br//handle/iec/2865xmlui.dri2xhtml.METS-1.0.item-author
Teixeira, Dielle Monteiro
Spada, Paula Katharine de Pontes
Morais, Lena Líllian Canto de Sá
Fumian, Tulio Machado
Lima, Ian Carlos Gomes de
Oliveira, Darleise de Souza
Bandeira, Renato da Silva
Gurjão, Tereza Cristina Monteiro
Sousa, Maísa Silva de
Mascarenhas, Joana D'Arc Pereira
Gabbay, Yvone Benchimol
xmlui.dri2xhtml.METS-1.0.item-abstract
This study investigated the presence of norovirus (NoV) GI and GII in environmental samples from the northern region of Brazil. Water samples were collected monthly (November 2008/October 2010) from different sources and sewage and concentrated by the adsorption-elution method. The NoV investigation used molecular methods followed by sequencing reactions. The general positivity for NoV was 33.9% (57/168). Considering the results obtained only in the semi-nested RT-PCR (reverse transcription polymerase chain reaction) and only in the TaqMan® real-time PCR, the rates were 26.8% (45/168) and 27.4% (46/168), respectively, being for NoV GI 22.2% (10/45) and 19.6% (9/46); for GII 17.8% (8/45) and 15.2% (7/46); and for GI + GII 60% (27/45) and 65.2% (30/46), respectively. Different GI (GI.1, GI.4, GI.7 and GI.8) and GII (GII.4, GII.6, GII.9, GII.12 and GII.14) genotypes were detected. These results demonstrated the NoV was disseminated in the waters of Belém city due to a lack of sanitation that allowed the discharge of contaminated effluents into these aquatic ecosystems.
xmlui.dri2xhtml.METS-1.0.item-citation
TEIXEIRA, Dielle Monteiro et al. Norovirus genogroups I and II in environmental water samples from Belém city, Northern Brazil. Journal of Water and Health, v. 15, n. 1, p. 163-174, Feb. 2017.xmlui.dri2xhtml.METS-1.0.item-decsPrimary
Norovirus / isolamento & purificaçãoNorovirus / genética
Água Doce / microbiologia
Esgotos / microbiologia
Filogenia
Genótipo
Análise de Sequência de RNA
Fases de Leitura Aberta
Monitoramento Ambiental / métodos
Reação em Cadeia da Polimerase em Tempo Real / métodos
Reação em Cadeia da Polimerase Via Transcriptase Reversa / métodos