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dc.contributor.authorTeixeira, Dielle Monteiro-
dc.contributor.authorSpada, Paula Katharine de Pontes-
dc.contributor.authorMorais, Lena Líllian Canto de Sá-
dc.contributor.authorFumian, Tulio Machado-
dc.contributor.authorLima, Ian Carlos Gomes de-
dc.contributor.authorOliveira, Darleise de Souza-
dc.contributor.authorBandeira, Renato da Silva-
dc.contributor.authorGurjão, Tereza Cristina Monteiro-
dc.contributor.authorSousa, Maísa Silva de-
dc.contributor.authorMascarenhas, Joana D'Arc Pereira-
dc.contributor.authorGabbay, Yvone Benchimol-
dc.date.accessioned2017-11-21T19:49:20Z-
dc.date.available2017-11-21T19:49:20Z-
dc.date.issued2017-
dc.identifier.citationTEIXEIRA, Dielle Monteiro et al. Norovirus genogroups I and II in environmental water samples from Belém city, Northern Brazil. Journal of Water and Health, v. 15, n. 1, p. 163-174, Feb. 2017.pt_BR
dc.identifier.issn1477-8920-
dc.identifier.urihttp://patua.iec.gov.br//handle/iec/2865-
dc.description.abstractThis study investigated the presence of norovirus (NoV) GI and GII in environmental samples from the northern region of Brazil. Water samples were collected monthly (November 2008/October 2010) from different sources and sewage and concentrated by the adsorption-elution method. The NoV investigation used molecular methods followed by sequencing reactions. The general positivity for NoV was 33.9% (57/168). Considering the results obtained only in the semi-nested RT-PCR (reverse transcription polymerase chain reaction) and only in the TaqMan® real-time PCR, the rates were 26.8% (45/168) and 27.4% (46/168), respectively, being for NoV GI 22.2% (10/45) and 19.6% (9/46); for GII 17.8% (8/45) and 15.2% (7/46); and for GI + GII 60% (27/45) and 65.2% (30/46), respectively. Different GI (GI.1, GI.4, GI.7 and GI.8) and GII (GII.4, GII.6, GII.9, GII.12 and GII.14) genotypes were detected. These results demonstrated the NoV was disseminated in the waters of Belém city due to a lack of sanitation that allowed the discharge of contaminated effluents into these aquatic ecosystems.pt_BR
dc.language.isoengpt_BR
dc.publisherIWA Publishingpt_BR
dc.rightsAcesso Abertopt_BR
dc.titleNorovirus genogroups I and II in environmental water samples from Belém city, Northern Brazilpt_BR
dc.typeArtigopt_BR
dc.subject.decsPrimaryNorovirus / isolamento & purificaçãopt_BR
dc.subject.decsPrimaryNorovirus / genéticapt_BR
dc.subject.decsPrimaryÁgua Doce / microbiologiapt_BR
dc.subject.decsPrimaryEsgotos / microbiologiapt_BR
dc.subject.decsPrimaryFilogeniapt_BR
dc.subject.decsPrimaryGenótipopt_BR
dc.subject.decsPrimaryAnálise de Sequência de RNApt_BR
dc.subject.decsPrimaryFases de Leitura Abertapt_BR
dc.subject.decsPrimaryMonitoramento Ambiental / métodospt_BR
dc.subject.decsPrimaryReação em Cadeia da Polimerase em Tempo Real / métodospt_BR
dc.subject.decsPrimaryReação em Cadeia da Polimerase Via Transcriptase Reversa / métodospt_BR
dc.coverage.temporalragefrom2008-
dc.coverage.temporalrageupto2010-
dc.creator.affilliationFederal University of Para State. Tropical Medicine Center. Postgraduate Program in Tropical Diseases. Belém, PA, Brazil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.pt_BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.pt_BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.pt_BR
dc.creator.affilliationOswaldo Cruz Foundation. Laboratory of Comparative and Environmental Virology. Rio de Janeiro, RJ, Brazil.pt_BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brazil.pt_BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.pt_BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.pt_BR
dc.creator.affilliationFederal University of Para State. Tropical Medicine Center. Belém, PA, Brazil.pt_BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.pt_BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.pt_BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.pt_BR
dc.identifier.doi10.2166/wh.2016.275.-


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