Biosynthesis, characterization and leishmanicidal activity of a biocomposite containing AgNPs-PVP-glucantime
Gélvez, Ana Patricia Cacua
Farias, Luis Henrique Seabra
Pereira, Victor Soares
Silva, Inaldo Claudio Martins da
Costa, Ana Carolina
Dias, Carmen Gilda Barroso Tavares
Costa, Roseane Maria Ribeiro
Silva, Silvia Helena Marques da
Rodrigues, Ana Paula Drummond
Development of functionalized nanocomposites containing AgNPs-PVP-Glucantime® to evaluate their leishmanicidal activity as a novel method for improving the pharmacological properties of the drug Glucantime® against extracellular promastigotes and intracellular amastigotes of Leishmania amazonensis in vitro to treat cutaneous leishmaniasis. Materials & methods: The silver nanoparticles and nanocomposites prepared containing silver nanoparticles, polyvinylpyrrolidone and different amounts of Glucantime were characterized using transmission electron microscopy, x-ray diffraction, energy-dispersive x-ray spectroscopy and ζ potential analysis; in addition, the in vitro cytotoxicity was evaluated. Results: The nanocomposites showed an inhibitory effect on the cellular viability of promastigote forms, with values of 47.06, 51.71 and 65.67% for nanocomposite1, nanocomposite2 and nanocomposite3, respectively, as well as a dose-dependent decrease in the infectivity index, with values of 33.33 and 23% for nanocomposite2 and nanocomposite3, respectively. Conclusion: The proposed nanocomposite reveals leishmanial activity and the absence of cytotoxicity in macrophages. Further investigations will be conducted in vivo.
xmlui.dri2xhtml.METS-1.0.item-citationGÉLVEZ, Ana Patricia Cacua et al. Biosynthesis, characterization and leishmanicidal activity of a biocomposite containing AgNPs-PVP-glucantime. Nanomedicine, v. 13, n. 4, p. 373-390, 2018.
xmlui.dri2xhtml.METS-1.0.item-decsPrimaryLeishmania / efeitos de drogas
Nanopartículas / biossíntese
Leishmaniose Cutânea / tratamento farmacológico