Molecular analysis of VP4, VP7, and NSP4 genes of PG2 rotavirus genotype strains recovered from neonates admitted to hospital in Belém, Brazil
Mascarenhas, Joana D'Arc Pereira
Linhares, Alexandre da Costa
Bayma, Amanda Patricia G
Lima, Jackson C
Sousa, Maisa S
Araújo, Irene T
Heinemann, Marcos B
Gusmão, Rosa Helena Porto
Gabbay, Yvone Benchimol
Leite, José Paulo G
This investigation describes the molecular characterization of P G2 rotavirus strains from hospitalized neonates with community-acquired diarrhea (CAD), nosocomial diarrhea (ND), and asymptomatic nosocomial infection (ANI) in Belém, Brazil. Twenty-six rotavirus strains with PG2 genotype were sequenced to genes coding for VP4, VP7, and NSP4 proteins. Phylogenetic analysis of the VP4 gene, including prototype strains RV3, ST3, M37, and U1205, showed that local P G2 strains clustered forming a distinct lineage (bootstrap of 99 per cent). Brazilian P G2 strains had the highest homology (ranging from 96.0 per cent -98.3 per cent) with the African strain GR1107, G4P. Phylogenetic tree for VP7 gene was constructed including old and new G2 African strains SA3958GR/97, SA356PT/96, SA514GR/87, SA4476PT/97, BF3676/99, GH1803/99, and representative strains of G1, G3, G4, G5, G8, and G9 genotypes. The Brazilian P G2 samples fell into a distinct group (bootstrap value of 97 per cent) and showed homology rates ranging from 92.1 per cent to 93.5 per cent with P G2 African strains BF3676/99, GH1803/99, and SA3958GR/97. Nucleotide sequence analysis of the NSP4 gene, including human prototype strains S2, KUN, DS-1, RV5, RV3 and ST3, and animal prototype OSU, showed that all neonatal isolates fell into genotype A and clustered with a bootstrap value of 100 per cent, with in-group similarities ranging from 99.3 per cent to 100 per cent. In this study no significant differences in nucleotide sequences of the VP4, VP7, and NSP4 genes could be observed when comparing diarrheic (CAD and ND) and non-diarrheic (ANI) babies. Monitoring of rotavirus strains in hospital environments is of particular importance, since it is claimed currently that an efficacious rotavirus vaccine, when available for routine use, will determine an impact on hospital-acquired rotavirus disease.
xmlui.dri2xhtml.METS-1.0.item-citationMASCARENHAS, Joana D’Arc Pereira et al. Molecular analysis of VP4, VP7, and NSP4 genes of PG2 rotavirus genotype strains recovered from neonates admitted to hospital in Belém, Brazil. Journal of Medical Virology, v. 78, p. 281-289, 2006.
xmlui.dri2xhtml.METS-1.0.item-decsPrimaryRotavirus / classificação