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Molecular characterization of human erythrovirus B19 strains obtained from patients with several clinical presentations in the Amazon region of Brazil.

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2008
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Molecular characterization of human erythrovirus B19 strains obtained from patients with several clinical presentations in the Amazon region of Brazil..pdf (313.2xmlui.dri2xhtml.METS-1.0.size-kilobytes)
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http://patua.iec.gov.br//handle/iec/3261
xmlui.dri2xhtml.METS-1.0.item-author
Freitas, Ronaldo Barros de
Melo, Fernando L
Oliveira, Darleise de Souza
Romano, Camila M
Freitas, Maria Rute Castro
Macêdo, Olinda
Linhares, Alexandre da Costa
Zanotto, Paolo M. de A
Durigon, Edison Luiz
xmlui.dri2xhtml.METS-1.0.item-abstract
Background: Human erythrovirus B19, endemic in the Amazon region since 1990, is associated with a wide spectrum of clinical presentations.Objectives: To assess the prevalence of erythrovirus B19 infection and the relative frequency of erythrovirus B19 genotypes in patients inthe Amazon region with various clinical presentations.Study design: A total of 487 clinical samples obtained from patients with symptoms suggestive of erythrovirus infection were tested usingspecific IgM and IgG antibody assays (ELISA) and PCR for viral DNA detection. Partial VP1 and VP2 regions were sequenced and genotyped by phylogenetic reconstruction.Results: B19 DNA was detected in 117 (24 por cento) of 487 samples. Of these, 106 (91 por cento) isolates were genotype 1 and 11 (9 por cento) were genotype 3.No genotype 2 was found. Genotype 1 had three clusters (A1, A2 and B) and all genotype 3 sequences were subtype 3b. All patients with hematological disorders within cluster B of genotype 1 were infected by the same B19 lineage, suggesting that this lineage of B19 may havebeen transmitted via transfusion of blood products.Conclusion: We reported two genotypes, 1 and 3b, with three genotype 1 clusters co-circulating in the Amazon region during the past 10 years.
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FREITAS, Ronaldo Barros de et al. Molecular characterization of human erythrovirus B19 strains obtained from patients with several clinical presentations in the Amazon region of Brazil. Journal of Clinical Virology, v. 43, p. 60+65, 2008.
xmlui.dri2xhtml.METS-1.0.item-decsPrimary
Erythrovirus / isolamento & purificação
Filogenia
Parvovirus B19 Humano
Eritema Infeccioso
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Acesso Aberto
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  • SAPAT - Artigos Científicos

Instituto Evandro Chagas - SVS - MS - 2007-2018 Rodovia BR316 km 7 sn - Levilandia - 67030-000 - Ananindeua - Para - Brasil.
Licença Creative CommonsEste trabalho está licenciado com uma Licença Creative Commons - Atribuição-NãoComercial 4.0 Internacional
Tel: (55 91) 3214-2191
Email: biblioteca@iec.gov.br / clariceneta@iec.gov.br
 

 

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Instituto Evandro Chagas - SVS - MS - 2007-2018 Rodovia BR316 km 7 sn - Levilandia - 67030-000 - Ananindeua - Para - Brasil.
Licença Creative CommonsEste trabalho está licenciado com uma Licença Creative Commons - Atribuição-NãoComercial 4.0 Internacional
Tel: (55 91) 3214-2191
Email: biblioteca@iec.gov.br / clariceneta@iec.gov.br