Phylogenetic analysis of probable non-human genes of group A rotaviruses isolated from children with acute gastroenteritis in Belém, Brazil

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2012xmlui.mirage2.itemSummaryView.MetaData
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http://patua.iec.gov.br//handle/iec/3268xmlui.dri2xhtml.METS-1.0.item-author
Maestri, Régis Piloni
Kaiano, Jane Haruko Lima
Neri, Darivaldo Luz
Soares, Luana da Silva
Guerra, Sylvia de Fátima dos Santos
Oliveira, Darleise de Souza
Farias, Yasmin Nascimento
Gabbay, Yvone Benchimol
Leite, José Paulo Gagliardi
Linhares, Alexandre da Costa
Mascarenhas, Joana D'Arc Pereira
xmlui.dri2xhtml.METS-1.0.item-abstract
Rotaviruses (RVs) are the main cause of acute viral gastroenteritis in both humans and young animals of various species such as calves, horses, pigs, dogs, cats, and birds. The genetic diversity of RVs is related to a variety of evolutionary mechanisms, including point mutation, and genome reassortment. The objective of this study was to characterize molecularly genes that encode structural and nonstructural proteins in unusual RV strains. The clinical specimens selected for this study were obtained from children and newborn with RV gastroenteritis, who participated in research projects on viral gastroenteritis conducted at the Evandro Chagas Institute. Structural (VP1-VP4, VP6, and VP7) and nonstructural (NSP1-NSP6) genes were amplified from stool samples by the polymerase chain reaction and subsequently sequenced. Eight unusual RV strains isolated from children and newborn with gastroenteritis were studied. Reassortment between genes of animal origin were observed in 5/8 (62.5%) strains analyzed. These results demonstrate that, although rare, interspecies (animal-human) transmission of RVs occurs in nature, as observed in the present study in strains NB150, HSP034, HSP180, HST327, and RV10109. This study is the first to be conducted in the Amazon region and supports previous data showing a close relationship between genes of human and animal origin, representing a challenge to the large-scale introduction of RV vaccines in national immunization programs.
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MAESTRI, Régis Piloni et al. Phylogenetic analysis of probable non-human genes of group A rotaviruses isolated from children with acute gastroenteritis in Belém, Brazil. Journal of Medical Virology, v. 84, n. 12, p. 1993-2002, 2012.xmlui.dri2xhtml.METS-1.0.item-decsPrimary
RotavirusInfecções por Rotavirus
Diarreia Infantil / virologia
Genética