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Ilheus virus (Flaviviridae, Flavivirus) is closely related to Japanese encephalitis virus complex

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1997
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Ilheus virus (Flaviviridae, Flavivirus) is closely related to Japanese encephalitis virus complex .pdf (1.407xmlui.dri2xhtml.METS-1.0.size-megabytes)
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http://patua.iec.gov.br/handle/iec/327
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Cruz, Ana Cecília Ribeiro
Rosa, Amélia Paes de Andrade Travassos da
Ferreira, Idevaldo I
Albuquerque, M. Matilde M
Galler, Ricardo
xmlui.dri2xhtml.METS-1.0.item-abstract
The Ilheus (ILH) virus has long been known to belong to group B of the arboviruses. Previous attempts to relate it to existing serogroups within the Flavivirus genus using conventional serological techniques such as hemagglutination inhibition, neutralization and complement fixation tests have been inconclusive. We have first used denaturing gel electrophoresis to estimate the molecular weight of immunoprecipitated radiolabeled viral proteins and the cross-reactivity among ILH proteins and hyperimmune sera to several flaviviruses only from the mosquito-borne encephalitis virus serogroups. The estimated molecular weight for the three proteins was in the same order of magnitude, as previously established, for mosquito-borne flaviviruses. Cross-immunoprecipitation tests showed that NS3 protein is the most cross-reactive. Partial nucleotide sequence analyses of the NS3 gene, corresponding to an area linking the helicase and the RNA triphosphatase domains, revealed that ILH virus is very closely related to the Japanese encephalitis virus complex confirming earlier serological data.
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CRUZ, Ana Cecilia Ribeiro et al. Ilheus virus (Flaviviridae, Flavivirus) is closely related to Japanese encephalitis virus complex. Intervirology, v. 40, n. 4, p. 220-225, July-Aug. 1997.
xmlui.dri2xhtml.METS-1.0.item-decsPrimary
Arbovirus / classificação
Flaviviridae / classificação
Vírus da Encefalite Japonesa (Espécie) / classificação
Vírus da Encefalite Japonesa (Espécie) / genética
Genoma Viral
Proteínas Virais / análise
Cercopithecus aethiops
Reações Cruzadas
RNA Helicases
Serina Endopeptidases
Células Vero
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Instituto Evandro Chagas - SVS - MS - 2007-2018 Rodovia BR316 km 7 sn - Levilandia - 67030-000 - Ananindeua - Para - Brasil.
Licença Creative CommonsEste trabalho está licenciado com uma Licença Creative Commons - Atribuição-NãoComercial 4.0 Internacional
Tel: (55 91) 3214-2191
Email: biblioteca@iec.gov.br / clariceneta@iec.gov.br
 

 

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Instituto Evandro Chagas - SVS - MS - 2007-2018 Rodovia BR316 km 7 sn - Levilandia - 67030-000 - Ananindeua - Para - Brasil.
Licença Creative CommonsEste trabalho está licenciado com uma Licença Creative Commons - Atribuição-NãoComercial 4.0 Internacional
Tel: (55 91) 3214-2191
Email: biblioteca@iec.gov.br / clariceneta@iec.gov.br