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dc.contributor.authorSiqueira, Jones Anderson Monteiro-
dc.contributor.authorLinhares, Alexandre da Costa-
dc.contributor.authorCarvalho, Thaís Cristina Nascimento de-
dc.contributor.authorAragão, Glicélia Cruz-
dc.contributor.authorOliveira, Darleise de Souza-
dc.contributor.authorSantos, Mirleide Cordeiro dos-
dc.contributor.authorSousa, Maisa Silvia de-
dc.contributor.authorJustino, Maria Cleonice Aguiar-
dc.contributor.authorMascarenhas, Joana D'Arc Pereira-
dc.contributor.authorGabbay, Yvone Benchimol-
dc.date.accessioned2018-07-19T17:32:23Z-
dc.date.available2018-07-19T17:32:23Z-
dc.date.issued2013-
dc.identifier.citationSIQUEIRA, Jones Anderson Monteiro et al. Norovirus infection in children admitted to hospital for acute gastroenteritis in Belém, Pará, Northern Brazil. Journal of Medical Virology, v. 85, p. 737-744, 2013.pt_BR
dc.identifier.issn0146-6615-
dc.identifier.urihttp://patua.iec.gov.br//handle/iec/3273
dc.description.abstractNoroviruses are the leading cause of epidemic, non-bacterial outbreaks of acute gastroenteritis, and are also a major cause of sporadic acute gastroenteritis in infants. The aim of the present study was to identify norovirus infections in children not infected by rotavirus admitted to hospital for acute gastroenteritis in Belém. A total of 348 fecal specimens were obtained from children with diarrhea aged less than 5 years, all of whom had tested negative for rotavirus, between May 2008 and April 2010. Fecal samples were screened for norovirus antigen using enzyme-immunoassay (EIA). Specimens were subjected to reverse-transcription polymerase chain reaction (RT-PCR) using the primers Mon432/434-Mon431/433 for detection of the GI and GII norovirus strains, respectively. Based on both methods, the overall norovirus positivity rate was 36.5% (127/348). Of the 169 samples collected in the first year, 44.4% (n = 75) tested positive for norovirus using both methods, 35.5% (n = 60) by EIA and 40.8% (n = 69) by RT-PCR. Using RT-PCR as a reference standard, a sensitivity of 78.3%, specificity of 94%, and agreement of 87.6% were recorded. Genome sequencing was obtained for 22 (31.9%) of the 69 positive samples, of which 90.9% (20/22) were genotype GII.4d and 9.1% (2/22) were genotype GII.b. Norovirus infection was most frequent in children under 2 years of age (41.5%-115/277). The peak incidence (62.1%) of norovirus-related acute gastroenteritis in these patients (not infected by rotavirus) was observed in February 2010. These findings emphasize the importance of norovirus as a cause of severe acute gastroenteritis among children in Belém, Pará, Northern Brazil.pt_BR
dc.description.sponsorshipCoordination of Improvement of Higher Education Personnel (CAPES) Evandro Chagas Institute, SVS, MSpt_BR
dc.language.isoengpt_BR
dc.publisherWileypt_BR
dc.rightsAcesso Abertopt_BR
dc.titleNorovirus infection in children admitted to hospital for acute gastroenteritis in Belém, Pará, Northern Brazilpt_BR
dc.typeArtigopt_BR
dc.subject.decsPrimaryGastroenterite / parasitologiapt_BR
dc.subject.decsPrimaryNoroviruspt_BR
dc.subject.decsPrimaryDiarreia Infantilpt_BR
dc.subject.decsPrimaryHospitais Públicospt_BR
dc.subject.decsPrimarySaúde Públicapt_BR
dc.creator.affilliationFederal University of Pará State. Tropical Medicine Center. Postgraduate Program in Tropical Diseases. Belém, PA, Brazil.pt_BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.pt_BR
dc.creator.affilliationFederal University of Pará State. Institute of Health Sciences. Belém, PA, Brazil.pt_BR
dc.creator.affilliationUniversity of the Pará State. Postgraduate Program in Parasite Biology in the Amazon, Center for Biological and Health Sciences. Belém, PA, Brazil.pt_BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.pt_BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.pt_BR
dc.creator.affilliationFederal University of Pará State. Tropical Medicine Center. Belém, PA, Brazil.pt_BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.pt_BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.pt_BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.pt_BR
dc.identifier.doi10.1002/jmv.23506-


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