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Evaluation of third-generation RIDASCREEN enzyme immunoassay for the detection of norovirus antigens in stool samples of hospitalized children in Belém, Pará, Brazil

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2011
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Evaluation of third-generation RIDASCREEN enzyme immunoassay for the detection of norovirus antigens in stool samples of hospitalized children in Belém, Pará, Brazil.pdf (302.2xmlui.dri2xhtml.METS-1.0.size-kilobytes)
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http://patua.iec.gov.br//handle/iec/3297
xmlui.dri2xhtml.METS-1.0.item-author
Siqueira, Jones Anderson Monteiro
Linhares, Alexandre da Costa
Oliveira, Darleise de Souza
Soares, Luana da Silva
Lucena, Maria Silvia Souza
Wanzeller, Ana Lúcia Monteiro
Mascarenhas, Joana D'Arc Pereira
Gabbay, Yvone Benchimol
xmlui.dri2xhtml.METS-1.0.item-abstract
Noroviruses (NoVs) are major agents of gastroenteritis outbreaks and hospitalization worldwide. This study evaluated the sensitivity and specificity of the commercially available third-generation RIDASCREEN® Norovirus Enzyme Immunoassay (EIA) kit in comparison to the reverse transcription-polymerase chain reaction (RT-PCR) to detect NoVs in hospitalized children with gastroenteritis. An agreement of 88% (81/92) was observed when comparing EIA with RT-PCR. A sensitivity of 92% and a specificity of 83.3% were demonstrated. Eleven samples were positive by 1 method only (4 RT-PCR/7 EIA). Fourteen samples were sequenced and all classified as NoV genogroup GII-4. The 7 positive only by EIA were also evaluated by electron microscopy, and in 3 (42.9%) samples viral particles with a suggestive morphology of NoVs were visualized. These same samples were tested by seminested-RT-PCR with a positivity of 85.7%. The results obtained in this study demonstrated a significant improvement in the sensitivity and specificity of this updated assay
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SIQUEIRA, Jones Anderson Monteiro et al. Evaluation of third-generation RIDASCREEN enzyme immunoassay for the detection of norovirus antigens in stool samples of hospitalized children in Belém, Pará, Brazil. Diagnostic Microbiology and Infectious Disease, v. 714, n. 4, p. 391 - 395, Dec. 2011.
xmlui.dri2xhtml.METS-1.0.item-decsPrimary
Norovirus / isolamento & purificação
Vírion / ultraestrutura
Infecções por Caliciviridae / diagnóstico
Infecções por Caliciviridae / virologia
Gastroenterite / diagnóstico
Gastroenterite / virologia
Antígenos Virais / análise
RNA Viral / genética
Análise de Sequência de DNA
Fezes / virologia
Sensibilidade e Especificidade
Genótipo
Técnicas Imunoenzimáticas / métodos
Microscopia Eletrônica de Transmissão
Reação em Cadeia da Polimerase Via Transcriptase Reversa / métodos
Criança Hospitalizada
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Instituto Evandro Chagas - SVS - MS - 2007-2018 Rodovia BR316 km 7 sn - Levilandia - 67030-000 - Ananindeua - Para - Brasil.
Licença Creative CommonsEste trabalho está licenciado com uma Licença Creative Commons - Atribuição-NãoComercial 4.0 Internacional
Tel: (55 91) 3214-2191
Email: biblioteca@iec.gov.br / clariceneta@iec.gov.br
 

 

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Instituto Evandro Chagas - SVS - MS - 2007-2018 Rodovia BR316 km 7 sn - Levilandia - 67030-000 - Ananindeua - Para - Brasil.
Licença Creative CommonsEste trabalho está licenciado com uma Licença Creative Commons - Atribuição-NãoComercial 4.0 Internacional
Tel: (55 91) 3214-2191
Email: biblioteca@iec.gov.br / clariceneta@iec.gov.br