xmlui.ArtifactBrowser.ItemViewer.show_simple

dc.contributor.authorPortal, Thayara Morais-
dc.contributor.authorReymão, Tammy Kathlyn Amaral-
dc.contributor.authorQuinderé Neto, Germano Alves-
dc.contributor.authorFiuza, Mercedes Kamila da C-
dc.contributor.authorTeixeira, Dielle Monteiro-
dc.contributor.authorLima, Ian Carlos Gomes-
dc.contributor.authorSousa Júnior, Edivaldo Costa-
dc.contributor.authorBandeira, Renato da Silva-
dc.contributor.authorDeus, Danielle Rodrigues de-
dc.contributor.authorJustino, Maria Cleonice Aguiar-
dc.contributor.authorLinhares, Alexandre da Costa-
dc.contributor.authorSilva, Luciana Damascena da-
dc.contributor.authorResque, Hugo Reis-
dc.contributor.authorGabbay, Yvone Benchimol-
dc.date.accessioned2018-11-13T19:46:19Z-
dc.date.available2018-11-13T19:46:19Z-
dc.date.issued2019-
dc.identifier.citationPORTAL, Thayara Morais et al. Detection and genotyping of enteric viruses in hospitalized children with acute gastroenteritis in Belém, Brazil: occurrence of adenovirus viremia by species F, types 40/41. Journal of Medical Virology, v. 91, n. 3, p. 378-384, Jan. 2019.pt_BR
dc.identifier.issn0146-6615-
dc.identifier.urihttp://patua.iec.gov.br//handle/iec/3529-
dc.description.abstractEnteric adenovirus (AdV), sapovirus (SaV), and human astrovirus (HAstV) are important pathogens involved in the gastroenteritis etiology. In this study, a total of 219 fecal samples and sera were collected from children hospitalized for acute gastroenteritis (AGE) in two large pediatric hospitals in Belém, from March 2012 to April 2015. The samples were analyzed by polymerase chain reaction (PCR) for adenovirus (AdV) and HAstV (astrovirus) detection, and Nested-PCR and qPCR for sapovirus (SaV) detection. AdV was detected in 50.2% (110/219) of the cases, with 42.7% (47/110) being sequenced and classified as: species F (63.9% - 30/47), A (4.2% - 2/47), B (6.4% - 3/47), C (17.1% - 8/47), D (4.2% - 2/47), and E (4.2% - 2/47). Of the 110 AdV-positive faeces samples, 80 paired sera presented sufficient amounts and were also tested for this virus, of which 51 (63.7%) showed positive results and 26 (70.3%) pairs (feces plus sera) presented concordant results after sequencing being classified as: species F (21/26; 80.8%), A (1/26; 3.8%), B (1/26; 3.8%), and C (3/26; 11.5%). Overall, HAstV rate in the feces samples was 1.8% (4/219), including both HAstV-1a (2/4; 50%) and HAstV-2c (2/4; 50%). SaV was detected in 4.6% (10/219) of the fecal samples, out of which 50% (5/10) of the positive samples were characterized into the genogroups GI.1 (1), GI.2 (2), and GII.4 (2). These findings highlighted the important contributions of AdV, HAstV, and SaV in the enteric virus spectrum in our region and showed the high genetic diversity of AdV. In addition, it demonstrated for the first time in Brazil, the circulation of AdV in the serum of hospitalized children with AGE.pt_BR
dc.description.sponsorshipThis research was supported by Evandro Chagas Institute; Capes.pt_BR
dc.language.isoengpt_BR
dc.publisherWileypt_BR
dc.rightsAcesso Abertopt_BR
dc.titleDetection and genotyping of enteric viruses in hospitalized children with acute gastroenteritis in Belém, Brazil: occurrence of adenovirus viremia by species F, types 40/41pt_BR
dc.typeArtigopt_BR
dc.subject.decsPrimaryGastroenterite / patologiapt_BR
dc.subject.decsPrimaryInfecções por Adenovirus Humanospt_BR
dc.subject.decsPrimaryAdenovírus Humanos / patogenicidadept_BR
dc.subject.decsPrimarySapoviruspt_BR
dc.subject.decsPrimaryMamastroviruspt_BR
dc.subject.decsPrimaryDiarreia Infantil / patologiapt_BR
dc.subject.decsPrimaryReação em Cadeia da Polimerase / métodospt_BR
dc.creator.affilliationUniversity of State of Pará. Postgraduate Program in Parasitic Biology in the Amazon. Belém, PA, Brazil.pt_BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas.Program de Pós-Graduação em Virologia. Ananindeua, PA, Brasil.pt_BR
dc.creator.affilliationUniversity of State of Pará. Undergraduate in Nursing. Belém, PA, Brazil.pt_BR
dc.creator.affilliationSchool of the Amazon. Belém, PA, Brazil.pt_BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.pt_BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.pt_BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.pt_BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.pt_BR
dc.creator.affilliationUniversity of State of Pará. Postgraduate Program in Parasitic Biology in the Amazon. Belém, PA, Brazil.pt_BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.pt_BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.pt_BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.pt_BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.pt_BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.pt_BR
dc.identifier.doi10.1002/jmv.25321-


xmlui.dri2xhtml.METS-1.0.item-files-head

Thumbnail

xmlui.ArtifactBrowser.ItemViewer.head_parent_collections

xmlui.ArtifactBrowser.ItemViewer.show_simple