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dc.contributor.authorMarinho, A. N. R-
dc.contributor.authorRocha, D. C. C-
dc.contributor.authorKanai, Y. K-
dc.contributor.authorAlves, C. M-
dc.contributor.authorCosta, D. C-
dc.contributor.authorSousa, A. H-
dc.contributor.authorBarros, B. C. V-
dc.contributor.authorBonfim, M. C. M. S-
dc.contributor.authorMascarenhas, Joana D'Arc Pereira-
dc.date.accessioned2019-02-20T18:00:29Z-
dc.date.available2019-02-20T18:00:29Z-
dc.date.issued2018-
dc.identifier.citationMARINHO, A. N. R. et al. Rotavirus analyses by SYBR Green real-time PCR and microbiological contamination in bivalves cultivated in coastal water of Amazonian Brazil. Journal of Water and Health, v. 16, n. 6 , p. 970-979, Dec. 2018.pt_BR
dc.identifier.issn1477-8920-
dc.identifier.urihttp://patua.iec.gov.br//handle/iec/3600-
dc.description.abstractThe contamination of mussels and oysters by viruses and bacteria is often associated with water contamination and gastroenteritis in humans. The present study evaluated viral and bacterial contamination in 380 samples, from nine mollusk-producing regions in coastal water north of the Brazilian Amazon. Rotavirus contamination was studied for groups A to H, using a two-step SYBR Green RT-qPCR (quantitative reverse transcription polymerase chain reaction), and bacterial families Enterobacteriaceae, Vibrionaceae, and Aeromonadaceae by classical and molecular methods. From the 19 pools analyzed, 26.3% (5/19) were positive for group A Rotavirus, I2 genotype for VP6 region, without amplifications for groups B–H. Bacteriological analysis identified Escherichia coli isolates in 89.5% (17/19) with identification of atypical enteropathogenic E. coli aEPEC in 10.5% (2/19), Salmonella (Groups C1 and G) (10.5%, 2/19), Vibrio alginolyticus (57.9%, 11/19) V. parahaemolyticus (63.2%, 12/19), V. fluvialis (42.1%, 8/19), V. vulnificus (10.5%, 2/19), V. cholerae non-O1, non O139(10.5%, 2/19) and Aeromonas salmonicida (52.6%, 10/19). All the samples investigated presented some level of contamination by enterobacteria, rotavirus, or both, and these results may reflect the level of contamination in the Northern Amazon Region, due to the natural maintenance of some of these agents or by the proximity with human populations and their sewer.pt_BR
dc.language.isoengpt_BR
dc.publisherIWA Publishingpt_BR
dc.rightsAcesso Abertopt_BR
dc.titleRotavirus analyses by SYBR Green real-time PCR and microbiological contamination in bivalves cultivated in coastal water of Amazonian Brazilpt_BR
dc.typeArtigopt_BR
dc.subject.decsPrimaryÁgua Costeira / análisept_BR
dc.subject.decsPrimaryPoluição Costeira / análisept_BR
dc.subject.decsPrimaryBivalves / crescimento & desenvolvimentopt_BR
dc.subject.decsPrimaryAnálise Microbiológicapt_BR
dc.subject.decsPrimaryContaminação de Alimentospt_BR
dc.subject.decsPrimaryMicrorganismos Aquáticos / análisept_BR
dc.subject.decsPrimaryReação em Cadeia da Polimerase em Tempo Real / métodospt_BR
dc.subject.decsPrimaryRegião Norte (BR)pt_BR
dc.subject.decsPrimaryAmazônia Brasileirapt_BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.pt_BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.pt_BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.pt_BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.pt_BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.pt_BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.pt_BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.pt_BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.pt_BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.pt_BR
dc.identifier.doi10.2166/wh.2018.130-


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