Minocycline treatment and bone marrow mononuclear cell transplantation after endothelin-1 induced striatal ischemia

Abstract

We explored whether the modulation of microglia activation with minocycline is beneficial to the therapeutic actions of bone marrow mononuclear cells (BMMCs) transplanted after experimental stroke. Male Wistar adult rats were divided in four experimental groups: ischemiccontrol saline treated (G1, N=6), ischemic minocycline treated (G2, N=5), ischemic BMMC treated (G3, N=5), and ischemic minocycline/BMMC treated (G4, N=6). There was a significant reduction in the number of ED1+ cells in G3 animals (51.31±2.41, P>0.05), but this effect was more prominentfollowing concomitant treatment with minocycline (G4=29.78±1.56). There was conspicuous neuronal preservation in the brains of G4 animals (87.97±4.27) compared with control group (G1=47.61±2.25, P>0.05). The behavioral tests showed better functional recovery in animals ofG2, G3, and G4, compared with G1 and baseline (P>0.05). The results suggest that a proper modulation of microglia activity may contribute to a more permissive ischemic environment contributing to increased neuroprotection and functional recovery following striatal ischemia.