Mechanical isolation of capuchin monkey (Cebus apella) preantral ovarian follicles
Domingues, Sheyla Farhayldes Souza
Ferreira, Humberto Soares
Muniz, José Augusto Pereira Carneiro
Lima, Ana Kelen Felipe
Ohashi, Otávio Mitio
Figueiredo, José Ricardo de
Silva, Lúcia Daniel Machado da
The aim of this study was to adapt a mechanical procedure for the isolation of intact preantral follicles from Cebus apella ovaries. The interval effect of serial sections of the tissue chopper was tested on a number of preantral follicles isolated from ovaries (n=6) of three C. apella females, two prepubertal and one adult. Ovaries were divided into four equal parts and fragmented with a tissue chopper, adjusted for serial sections at intervals of 250, 500, 750 and 1,000µm, respectively. Isolated follicles were counted in a Neubauer's chamber and classified as primordial, primary or secondary. The number (mean±SE) of preantral follicles isolated from 1/4 ovary varied from 68,330+17,590 (at the 1,000µm cut interval) to 300,830+111,460 (at the 500µm cut interval. The mean diameter of the isolated preantral follicles varied from 11.6µm to 27.8µm.
xmlui.dri2xhtml.METS-1.0.item-citationDOMINGUES, Sheyla Farhayldes Souza et al. Mechanical isolation of capuchin monkey (Cebus apella) preantral ovarian follicles. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, v. 55, n. 3, p. 301-308, jun. 2003.
xmlui.dri2xhtml.METS-1.0.item-decsPrimaryPrimatas / anatomia & histologia
Cebus / anatomia & histologia
Folículo Ovariano / citologia
Ovário / anatomia & histologia