Mechanical isolation of capuchin monkey (Cebus apella) preantral ovarian follicles

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2003xmlui.dri2xhtml.METS-1.0.item-files-viewOpen
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http://patua.iec.gov.br//handle/iec/3789xmlui.dri2xhtml.METS-1.0.item-author
Domingues, Sheyla Farhayldes Souza
Ferreira, Humberto Soares
Muniz, José Augusto Pereira Carneiro
Lima, Ana Kelen Felipe
Ohashi, Otávio Mitio
Figueiredo, José Ricardo de
Silva, Lúcia Daniel Machado da
xmlui.dri2xhtml.METS-1.0.item-abstract
The aim of this study was to adapt a mechanical procedure for the isolation of intact preantral follicles from Cebus apella ovaries. The interval effect of serial sections of the tissue chopper was tested on a number of preantral follicles isolated from ovaries (n=6) of three C. apella females, two prepubertal and one adult. Ovaries were divided into four equal parts and fragmented with a tissue chopper, adjusted for serial sections at intervals of 250, 500, 750 and 1,000µm, respectively. Isolated follicles were counted in a Neubauer's chamber and classified as primordial, primary or secondary. The number (mean±SE) of preantral follicles isolated from 1/4 ovary varied from 68,330+17,590 (at the 1,000µm cut interval) to 300,830+111,460 (at the 500µm cut interval. The mean diameter of the isolated preantral follicles varied from 11.6µm to 27.8µm.
xmlui.dri2xhtml.METS-1.0.item-citation
DOMINGUES, Sheyla Farhayldes Souza et al. Mechanical isolation of capuchin monkey (Cebus apella) preantral ovarian follicles. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, v. 55, n. 3, p. 301-308, jun. 2003.xmlui.dri2xhtml.METS-1.0.item-decsPrimary
Primatas / anatomia & histologiaCebus / anatomia & histologia
Folículo Ovariano / citologia
Isolamento Reprodutivo
Ovário / anatomia & histologia