xmlui.ArtifactBrowser.ItemViewer.show_simple

dc.contributor.authorGomes, Fátima-
dc.contributor.authorWatanabe, Luciana-
dc.contributor.authorVianez Júnior, João Lídio da Silva Gonçalves-
dc.contributor.authorNunes, Márcio Roberto Teixeira-
dc.contributor.authorCardoso, Jedson Ferreira-
dc.contributor.authorLima, Clayton-
dc.contributor.authorSchneider, Horacio-
dc.contributor.authorSampaio, Iracilda-
dc.date.accessioned2019-08-14T14:26:18Z-
dc.date.available2019-08-14T14:26:18Z-
dc.date.issued2019-
dc.identifier.citationGOMES, Fátima et al. Comparative analysis of the transcriptome of the Amazonian fish species Colossoma macropomum (tambaqui) and hybrid tambacu by next generation sequencing. PLoS ONE, v. 14, n. 2, e0212755, p. 1-23, Feb. 2019.pt_BR
dc.identifier.issn1932-6203-
dc.identifier.urihttp://patua.iec.gov.br//handle/iec/3834-
dc.description.abstractBACKGROUND: The C. macropomum is a characiform fish from the Amazon basin that has been hybridized with other pacu species to produce commercial hybrids, such as the tambacu. However, little is known of the functional genomics of the parental species or these hybrid forms. The transcriptome of C. macropomum and tambacu were sequenced using 454 Roche platform (pyrosequencing) techniques to characterize the domains of Gene Ontology (GO) and to evaluate the levels of gene expression in the two organisms. RESULTS: The 8,188,945 reads were assembled into 400,845 contigs. A total of 58,322 contigs were annotated with a predominance of biological processes for both organisms, as determined by Gene Ontology (GO). Similar numbers of metabolic pathways were identified in both the C. macropomum and the tambacu, with the metabolism category presenting the largest number of transcripts. The BUSCO analysis indicated that our assembly was more than 40% complete. We identified 21,986 genes for the two fishes. The P and Log2FC values indicated significant differences in the levels of gene expression, with a total of 600 up-regulated genes. CONCLUSION: In spite of the lack of a reference genome, the functional annotation was successful, and confirmed a considerable difference in the specificity and levels of gene expression between the two organisms. This report provides a comprehensive baseline for the genetic management of these commercially important fishes, in particular for the identification of specific genes that may represent markers involved in the immunity, growth, and fertility of these organisms, with potential practical applications in aquaculture management.pt_BR
dc.description.sponsorshipCenter of Technological Innovation (CIT), Evandro Chagas Institute (IEC) and National Council of Scientific and Technological Development (CNPq) (Bionorte Project: 550939/2010-5).pt_BR
dc.language.isoengpt_BR
dc.publisherPublic Library of Sciencept_BR
dc.rightsAcesso Abertopt_BR
dc.titleComparative analysis of the transcriptome of the Amazonian fish species Colossoma macropomum (tambaqui) and hybrid tambacu by next generation sequencingpt_BR
dc.typeArtigopt_BR
dc.subject.decsPrimaryPeixes / genéticapt_BR
dc.subject.decsPrimaryOntologia Genéticapt_BR
dc.subject.decsPrimaryCaraciformes / genéticapt_BR
dc.subject.decsPrimaryColossoma macropomumpt_BR
dc.subject.decsPrimaryTambaquipt_BR
dc.subject.decsPrimaryManejo de Espécimespt_BR
dc.subject.decsPrimaryAquiculturapt_BR
dc.creator.affilliationUniversidade Federal do Pará - Campus de Bragança. Institute of Coastal Studies. Laboratory of Genetics and Molecular Biology. Bragança, PA, Brazil.pt_BR
dc.creator.affilliationUniversidade Federal do Pará - Campus de Bragança. Institute of Coastal Studies. Laboratory of Genetics and Molecular Biology. Bragança, PA, Brazil.pt_BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.pt_BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.pt_BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Programa de Pós-Graduação em Virologia. Ananindeua, PA, Brasil.pt_BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.pt_BR
dc.creator.affilliationUniversidade Federal do Pará - Campus de Bragança. Institute of Coastal Studies. Laboratory of Genetics and Molecular Biology. Bragança, PA, Brazil.pt_BR
dc.creator.affilliationUniversidade Federal do Pará - Campus de Bragança. Institute of Coastal Studies. Laboratory of Genetics and Molecular Biology. Bragança, PA, Brazil.pt_BR
dc.identifier.doi10.1371/journal.pone.0212755-


xmlui.dri2xhtml.METS-1.0.item-files-head

Thumbnail

xmlui.ArtifactBrowser.ItemViewer.head_parent_collections

xmlui.ArtifactBrowser.ItemViewer.show_simple