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Oropouche orthobunyavirus: genetic characterization of full-length genomes and development of molecular methods to discriminate natural reassortments

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2019
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Oropouche orthobunyavirus: genetic characterization of full-length genomes and development of molecular methods to discriminate natural reassortments.pdf (538.1xmlui.dri2xhtml.METS-1.0.size-kilobytes)
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http://patua.iec.gov.br//handle/iec/3837
xmlui.dri2xhtml.METS-1.0.item-author
Nunes, Márcio Roberto Teixeira
Souza, William Marciel de
Savji, Nazir
Figueiredo, Mário Luís
Cardoso, Jedson Ferreira
Silva, Sandro Patroca da
Lima, Clayton Pereira Silva de
Vasconcelos, Helena Baldez
Rodrigues, Sueli Guerreiro
Lipkin, W. Ian
Vasconcelos, Pedro Fernando da Costa
Palacios, Gustavo
xmlui.dri2xhtml.METS-1.0.item-abstract
Oropouche orthobunyavirus (OROV) has significant impact in public health in Amazon region. This arbovirus is one of the most common causes of febrile illness in Brazil, and is responsible for several epidemics since 1960's. In this study, we sequenced and characterized the complete coding sequences (S-, M- and L-RNA) of 35 OROV isolates from Brazil. Here, we classified 20 strains in genotype I from Pará and Maranhão states, nine as genotype II from Pará and Rondônia states confirmed, four classified into genotype III from Acre, Maranhão, Minas Gerais and Rondônia states and two genotype IV from Amazonas State. Also, we did not observe reassortment events involving the OROV isolates. In addition, we developed novel RT-PCR tools to identify reassortment events among OROV strains. These data will be useful to better understand the molecular epidemiology and diagnostic of OROV infections.
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NUNES, Márcio Roberto Teixeira et al. Oropouche orthobunyavirus: genetic characterization of full-length genomes and development of molecular methods to discriminate natural reassortments. Infection, Genetics and Evolution, v. 68, p. 16-22, Mar. 2019.
xmlui.dri2xhtml.METS-1.0.item-decsPrimary
Arbovirus / isolamento & purificação
Orthobunyavirus / patogenicidade
Arbovírus / genética
Genótipo
Reação em Cadeia da Polimerase / métodos
Rearranjo Gênico
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Instituto Evandro Chagas - SVS - MS - 2007-2018 Rodovia BR316 km 7 sn - Levilandia - 67030-000 - Ananindeua - Para - Brasil.
Licença Creative CommonsEste trabalho está licenciado com uma Licença Creative Commons - Atribuição-NãoComercial 4.0 Internacional
Tel: (55 91) 3214-2191
Email: biblioteca@iec.gov.br / clariceneta@iec.gov.br
 

 

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Instituto Evandro Chagas - SVS - MS - 2007-2018 Rodovia BR316 km 7 sn - Levilandia - 67030-000 - Ananindeua - Para - Brasil.
Licença Creative CommonsEste trabalho está licenciado com uma Licença Creative Commons - Atribuição-NãoComercial 4.0 Internacional
Tel: (55 91) 3214-2191
Email: biblioteca@iec.gov.br / clariceneta@iec.gov.br