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dc.contributor.authorAcrani, Gustavo Olszanski-
dc.contributor.authorTilston-Lunel, Natasha L-
dc.contributor.authorSpiegel, Martin-
dc.contributor.authorWeidmann, Manfred-
dc.contributor.authorDilcher, Meik-
dc.contributor.authorSilva, Daisy Elaine Andrade da-
dc.contributor.authorNunes, Márcio Roberto Teixeira-
dc.contributor.authorElliott, Richard M-
dc.date.accessioned2019-08-28T12:03:20Z-
dc.date.available2019-08-28T12:03:20Z-
dc.date.issued2015-
dc.identifier.citationACRANI, Gustavo Olszanski et al. Establishment of a minigenome system for Oropouche virus reveals the S genome segment to be significantly longer than reported previously. Journal of General Virology, v. 96, pt. 3, p. 513-523, Mar. 2015.pt_BR
dc.identifier.issn1465-2099-
dc.identifier.urihttp://patua.iec.gov.br//handle/iec/3858-
dc.description.abstractOropouche virus (OROV) is a medically important orthobunyavirus, which causes frequent outbreaks of a febrile illness in the northern parts of Brazil. However, despite being the cause of an estimated half a million human infections since its first isolation in Trinidad in 1955, details of the molecular biology of this tripartite, negative-sense RNA virus remain limited. We have determined the complete nucleotide sequence of the Brazilian prototype strain of OROV, BeAn 19991, and found a number of differences compared with sequences in the database. Most notable were that the S segment contained an additional 204 nt at the 3' end and that there was a critical nucleotide mismatch at position 9 within the base-paired terminal panhandle structure of each genome segment. In addition, we obtained the complete sequence of the Trinidadian prototype strain TRVL-9760 that showed similar characteristics to the BeAn 19991 strain. By using a T7 RNA polymerase-driven minigenome system, we demonstrated that cDNA clones of the BeAn 19991 L and S segments expressed functional proteins, and also that the newly determined terminal untranslated sequences acted as functional promoters in the minigenome assay. By co-transfecting a cDNA to the viral glycoproteins, virus-like particles were generated that packaged a minigenome and were capable of infecting naive cells.pt_BR
dc.description.sponsorshipWellcome Trust Senior Investigator Award to R. M. E. (099220), Medical Research Council postgraduate studentship to N. L. T.-L., FAPESP-Sao Paulo Research Foundation fellowship to G. O. A. (2013/02798-0, CNPQ) and CAPES-National Council for the Improvement of Higher Education (Brazil) scholarship to D. E. A. S. (3851/10-9).pt_BR
dc.language.isoengpt_BR
dc.publisherMicrobiology Societypt_BR
dc.rightsAcesso Abertopt_BR
dc.titleEstablishment of a minigenome system for Oropouche virus reveals the S genome segment to be significantly longer than reported previouslypt_BR
dc.typeArtigopt_BR
dc.subject.decsPrimaryOrthobunyavirus / genéticapt_BR
dc.subject.decsPrimaryGenoma Viralpt_BR
dc.subject.decsPrimaryClonagem Molecularpt_BR
dc.subject.decsPrimaryGenômica / métodospt_BR
dc.subject.decsPrimaryDados de Sequência Molecularpt_BR
dc.subject.decsPrimaryRNA Viral / genéticapt_BR
dc.creator.affilliationUniversity of Glasgow. Centre for Virus Research Glasgow. Scotland, UK / University of São Paulo. School of Medicine. Department of Cell and Molecular Biology. Ribeirão Preto, SP, Brazil.pt_BR
dc.creator.affilliationUniversity of Glasgow. Centre for Virus Research Glasgow. Scotland, UK / University of St Andrews. School of Biology. Biomedical Sciences Research Complex. St Andrews, Scotland, UK.pt_BR
dc.creator.affilliationUniversity Medical Center Göttingen. Department of Virology. Gottingen, Germany.pt_BR
dc.creator.affilliationUniversity Medical Center Göttingen. Department of Virology. Gottingen, Germany.pt_BR
dc.creator.affilliationUniversity Medical Center Göttingen. Department of Virology. Gottingen, Germany.pt_BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.pt_BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.pt_BR
dc.creator.affilliationUniversity Medical Center Göttingen. Department of Virology. Gottingen, Germany.pt_BR
dc.identifier.doi10.1099/jgv.0.000005-


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