Molecular characterization of Babesia vogeli in dogs from Belém, northern Brazil

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2015xmlui.dri2xhtml.METS-1.0.item-files-viewOpen
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http://patua.iec.gov.br//handle/iec/3864xmlui.dri2xhtml.METS-1.0.item-author
Moraes, Pablo H. G
Rufino, C. P
Baraúna, A. R. F
Reis, T
Agnol, L. T. D
Meneses, A. M. C
Aguiar, Délia Cristina Figueira
Nunes, Marcio Roberto Teixeira
Gonçalves, Evonnildo Costa
xmlui.dri2xhtml.METS-1.0.item-abstract
Babesiosis is an infectious hemolytic disease that occurs worldwide, and is caused by a protozoan of the Babesia genus (Apicomplexa). Little is known about this genus; therefore, this study conducted a molecular characterization of Babesia spp in naturally infected dogs in northern Brazil. Blood samples were collected from 172 dogs from metropolitan Belém, and screened for Babesia spp using semi-nested polymerase chain reactions. Subsequently, 18S rDNA gene fragments were amplified and sequenced. Alignments of the 27 nucleotide sequences returned fragments measuring 1023 bp in size, which did not include any polymorphic sites (100 per cent identity). This genotype was very similar to the 18S rDNA gene in B. vogeli. This study provides an important molecular characterization of the parasite responsible for canine babesiosis in naturally infected dogs in metropolitan Belém. In addition, the 18S rDNA gene in B. vogeli may represent the most common genotype occurring in South America.
xmlui.dri2xhtml.METS-1.0.item-citation
MORAES, Pablo H. G. et al. Molecular characterization of Babesia vogeli in dogs from Belém, northern Brazil. Genetics and Molecular Research. (Online), v. 14, n. 4, p. 16364-16371, Dec. 2015.xmlui.dri2xhtml.METS-1.0.item-decsPrimary
Babesiose / parasitologiaBabesiose / epidemiologia
Doenças do Cão / parasitologia
Doenças do Cão / epidemiologia
Doenças Transmitidas por Carrapatos
Babesia / isolamento & purificação
Rhipicephalus sanguineus
Análise de Sequência de DNA
Reação em Cadeia da Polimerase / métodos