Comparison of semen characteristics and sperm cryopreservation in common marmoset (Callithrix jacchus) and black-tufted- ear marmoset (Callithrix penicillata)
Arakaki, Paloma Rocha
Monteiro, Frederico Ozanan Barros
Muniz, José Augusto Pereira Carneiro
Guimarães, Marcelo Alcindo de Barros Vaz
Valle, Rodrigo del Rio do
Background: The common marmoset (Callithrix jacchus) is one of the most studied Neotropical primates regarding reproduction. However, little has been reported on the black-tufted- ear marmoset (Callithrix penicillata), which may produce fertile hybrids with other Callithrix. This is the first study to evaluate sperm freezing from black-tufted- ear marmoset. Methods: Testicles from all animals were measured, and semen was collected by penile vibrostimulation. Samples were analyzed after collection, after chilling and addition of glycerol, and after thawing. Results and Conclusions: Fresh semen from both species was similar in many aspects. Additionally, there is a relationship between total motility, plasma membrane integrity, and acrosome integrity observed in sperm samples from both species. We managed to evaluate fresh and thawed sperm with suitable methods for use under zoo or field conditions. However, TEST egg yolk with glycerol at 4% and 6% concentration was not effective for sperm protection in both species during cryopreservation.
ReferenciaARAKAKI, Paloma Rocha et al. Comparison of semen characteristics and sperm cryopreservation in common marmoset (Callithrix jacchus) and black-tufted- ear marmoset (Callithrix penicillata). Journal of Medical Primatology, v. 48, n, 1, p. 32-42, Feb. 2019.
DeCsPrimatas / anatomia & histologia
Callithrix / anatomia & histologia
Reprodução / genética
Preservação do Sêmen / veterinária
Análise do Sêmen / veterinária