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Cooling and freezing of sperm from captive, free-living and endangered squirrel monkey species

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2016
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Cooling and freezing of sperm from captive, free-living and endangered squirrel monkey species.pdf (1.256xmlui.dri2xhtml.METS-1.0.size-megabytes)
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http://patua.iec.gov.br//handle/iec/4023
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Oliveira, Karol G
Santos, Regiane R
Leão, Danuza L
Brito, Adriel B
Lima, Julianne S
Sampaio, Wlaísa V
Domingues, Sheyla Farhayldes Souza
xmlui.dri2xhtml.METS-1.0.item-abstract
Germoplasm banking is an important tool for the preservation of genetic material from Neotropical primates in captivity, and from free living species, especially the endangered ones like Saimiri vanzolinii (Black-headed squirrel monkey), a primate with a low incidence area (870 km2 of floodplains) in the southern part of the Mamirau a Sustainable Development Reserve, Brazil. Therefore, in the present study we aimed to develop a sperm cryopreservation protocol comparing sperm cooling in presence (T1) and absence (T2) of egg yolk, and to test freezing protocols to preserve semen from captive (Saimiri collinsi), and free-living (Saimiri vanzolinii, Saimiri cassiquiarensis and Saimiri macrodon) New World primates. Cooling preserved sperm of S. collinsi in all evaluated microscopic parameters, except for sperm motility. No differences were observed among the treatments, indicating that semen of this species can be cooled without egg yolk. Freezing did not affect sperm quality of S. collinsi, except plasma membrane integrity that was negatively affected. Generally, a good maintenance rate was observed between cooling and thawing of semen for the four species, showing the positive translational application of protocols from S. collinsi to the free-living species. Developed freezing protocol proved to be useful for sperm cryopreservation of S. collinsi and in field conditions.
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OLIVEIRA, Karol G. et al. Cooling and freezing of sperm from captive, free-living and endangered squirrel monkey species. Cryobiology, v. 72, n. 3, p. 283-289, June 2016.
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Primatas / anatomia & histologia
Saimiri / anatomia & histologia
Preservação do Sêmen / veterinária
Bancos de Esperma
Criopreservação / veterinária
Espécies em Perigo de Extinção
Reserva de Desenvolvimento Sustentável Mamirauá (AM)
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Acesso Aberto
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  • CENP - Artigos Científicos

Instituto Evandro Chagas - SVS - MS - 2007-2018 Rodovia BR316 km 7 sn - Levilandia - 67030-000 - Ananindeua - Para - Brasil.
Licença Creative CommonsEste trabalho está licenciado com uma Licença Creative Commons - Atribuição-NãoComercial 4.0 Internacional
Tel: (55 91) 3214-2191
Email: biblioteca@iec.gov.br / clariceneta@iec.gov.br
 

 

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Instituto Evandro Chagas - SVS - MS - 2007-2018 Rodovia BR316 km 7 sn - Levilandia - 67030-000 - Ananindeua - Para - Brasil.
Licença Creative CommonsEste trabalho está licenciado com uma Licença Creative Commons - Atribuição-NãoComercial 4.0 Internacional
Tel: (55 91) 3214-2191
Email: biblioteca@iec.gov.br / clariceneta@iec.gov.br