xmlui.ArtifactBrowser.ItemViewer.show_simple

dc.contributor.authorScalercio, Sarah Raphaella Rocha de Azevedo-
dc.contributor.authorBrito, A. B-
dc.contributor.authorDomingues, Sheyla Farhayldes Souza-
dc.contributor.authorSantos, R. R-
dc.contributor.authorAmorim, C. A-
dc.date.accessioned2020-02-12T19:16:25Z-
dc.date.available2020-02-12T19:16:25Z-
dc.date.issued2015-
dc.identifier.citationSCALERCIO, Sarah Raphaella Rocha de Azevedo et al. Immunolocalization of growth, inhibitory, and proliferative factors involved in initial ovarian folliculogenesis from adult common squirrel monkey (Saimiri collinsi). Reproductive Sciences, v. 22, n. 1, p. 68-74, 2015.pt_BR
dc.identifier.issn1933-7205-
dc.identifier.urihttp://patua.iec.gov.br//handle/iec/4028-
dc.description.abstractWe performed an immunohistochemical (IHC) study to determine the follicular expression of growth differentiation factor 9 (GDF-9), anti-Mu¨llerian hormone (AMH), Kit Ligand (KL), and c-Kit in squirrel monkey ovary. Ovarian tissue fragments from 4 squirrel monkeys were collected by laparotomy and processed for classical histology and IHC. Additionally, follicle development was assessed by Ki67 immunostaining to evaluate proliferative status of granulosa cells. A total of 4025 follicles were examined (1475 for classical histology and 2550 for immunohistochemistry). More than 80% of the evaluated follicles were morphologically normal. The GDF-9 protein was detectable in oocyte cytoplasm from primordial (100%), primary (99.1%), and secondary (100%) follicles. The AMH was not expressed in primordial follicles but just in few primary follicles (13.8%). On the other hand, it was highly expressed in granulosa cells from secondary follicles (67.9%). c-Kit, KL receptor, was found in the oolemma of primordial (100%), primary (100%), and secondary (100%) follicles. The KL expression was observed in oocytes and granulosa cells from primordial (94.9%), primary (91.6%) and secondary follicles (100%). Ki67 immunostaining was observed in granulosa cells from primary (5.7%) and secondary (54.8%) follicles but not in primordial follicles. In conclusion, we described the localization of GDF-9, KL, c-Kit, and Ki67 proteins and confirmed the presence of AMH protein in preantral follicles from squirrel monkey. Our results offer contribution for understanding of folliculogenesis in neotropical nonhuman primates. Moreover, these markers can be used to assess follicular viability and functionality after cryopreservation, transplantation, or in vitro culture of ovarian tissue.pt_BR
dc.description.sponsorshipCapespt_BR
dc.language.isoengpt_BR
dc.publisherSAGE Publicationspt_BR
dc.rightsAcesso Abertopt_BR
dc.titleImmunolocalization of growth, inhibitory, and proliferative factors involved in initial ovarian folliculogenesis from adult common squirrel monkey (Saimiri collinsi)pt_BR
dc.typeArtigopt_BR
dc.subject.decsPrimaryPrimatas / anatomia & histologiapt_BR
dc.subject.decsPrimarySaimiri / anatomia & histologiapt_BR
dc.subject.decsPrimaryOvário / anatomia & histologiapt_BR
dc.subject.decsPrimaryFolículo Ovariano / ultraestruturapt_BR
dc.subject.decsPrimaryProliferação de Célulaspt_BR
dc.creator.affilliationFederal University of Pará. Laboratory of Wild Animal Biology and Medicine. Castanhal, PA, Brazil / Federal University of Pará. Animal Sciences PhD Program. Belém, PA, Brazil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Centro Nacional de Primatas. Ananindeua, PA, Brasil.pt_BR
dc.creator.affilliationFederal University of Pará. Laboratory of Wild Animal Biology and Medicine. Castanhal, PA, Brazil / Federal University of Pará. Animal Sciences PhD Program. Belém, PA, Brazil.pt_BR
dc.creator.affilliationFederal University of Pará. Laboratory of Wild Animal Biology and Medicine. Castanhal, PA, Brazil / Federal University of Pará. Animal Sciences PhD Program. Belém, PA, Brazil.pt_BR
dc.creator.affilliationFederal University of Pará. Laboratory of Wild Animal Biology and Medicine. Castanhal, PA, Brazil / Federal University of Pará. Animal Sciences PhD Program. Belém, PA, Brazil / Utrecht University. Faculty of Veterinary Medicine. Utrecht, the Netherlands.pt_BR
dc.creator.affilliationUniversité´ Catholique de Louvain. Institut de Recherche Expérimentale et Clinique. Pôle de Recherche en Gynécologie. Brussels, Belgium.pt_BR
dc.identifier.doi10.1177/1933719114532842-


xmlui.dri2xhtml.METS-1.0.item-files-head

Thumbnail

xmlui.ArtifactBrowser.ItemViewer.head_parent_collections

xmlui.ArtifactBrowser.ItemViewer.show_simple