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dc.contributor.authorScalercio, Sarah Raphaella Rocha de Azevedo-
dc.contributor.authorAmorim, Christiani A-
dc.contributor.authorBrito, Danielle C-
dc.contributor.authorPercário, Sandro-
dc.contributor.authorOskam, Irma C-
dc.contributor.authorDomingues, Sheyla Farhayldes Souza-
dc.contributor.authorSantos, Regiane R-
dc.date.accessioned2020-02-13T17:01:12Z-
dc.date.available2020-02-13T17:01:12Z-
dc.date.issued2016-
dc.identifier.citationSCALERCIO, Sarah Raphaella Rocha de Azevedo et al. Trolox enhances follicular survival after ovarian tissue autograft in squirrel monkey (Saimiri collinsi). Reproduction, Fertility and Development, v. 28, n. 11, p. 1854-1864, 2016.pt_BR
dc.identifier.issn1448-5990-
dc.identifier.urihttp://patua.iec.gov.br//handle/iec/4029-
dc.description.abstractThe aim of this study was to evaluate ovarian tissue pre-treatment with 50 µM Trolox followed by heterotopic transplantation in squirrel monkeys (Saimiri collinsi) and to assess tissue functionality via immunohistochemical analysis of the stroma and ovarian follicles. Five healthy and sexually mature squirrel monkey (Saimiri collinsi) females were used. Heterotopic autografting of fresh ovarian tissue with or without previous exposure to the antioxidant Trolox was performed and grafts were recovered for analysis 7 days later. Tissue vascularisation was confirmed by both macroscopic inspection and cluster of differentiation 31 (CD31) staining. Trolox prevented massive follicular activation and kept the percentages of morphologically normal follicles higher than in untreated grafts. Expression of anti-Müllerian hormone in developing follicles was observed only in controls and Trolox-treated grafts. Also, immunostaining for growth differentiation factor-9 was positive only in primordial follicles from controls and from Trolox-treated grafts. Although Trolox improved follicular quality and avoided apoptosis in stromal cells, ovarian tissue fibrosis was increased in Trolox-treated grafts, mainly due to an increase in collagen Type I synthesis.pt_BR
dc.description.sponsorshipCapespt_BR
dc.language.isoengpt_BR
dc.publisherCSIRO Publishingpt_BR
dc.rightsAcesso Abertopt_BR
dc.titleTrolox enhances follicular survival after ovarian tissue autograft in squirrel monkey (Saimiri collinsi)pt_BR
dc.typeArtigopt_BR
dc.subject.decsPrimaryPrimatas / anatomia & histologiapt_BR
dc.subject.decsPrimarySaimiri / anatomia & histologiapt_BR
dc.subject.decsPrimaryOvário / anatomia & histologiapt_BR
dc.subject.decsPrimaryTransplante / veterináriapt_BR
dc.subject.decsPrimaryTécnicas Reprodutivas / veterináriapt_BR
dc.subject.decsPrimaryVascularizaçãopt_BR
dc.creator.affilliationFederal University of Pará. Laboratory of Wild Animal Biology and Medicine. Castanhal, PA, Brazil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Centro Nacional de Primatas. Ananindeua, PA, Brasil.pt_BR
dc.creator.affilliationUniversité Catholique de Louvain. Institut de Recherche Expérimentale et Clinique. Pôle de Recherche en Gynécologie. Brussels, Belgium.pt_BR
dc.creator.affilliationFederal University of Pará. Laboratory of Wild Animal Biology and Medicine. Castanhal, PA, Brazil.pt_BR
dc.creator.affilliationFederal University of Pará. Oxidative Stress Research Laboratory. Belém, PA, Brazil.pt_BR
dc.creator.affilliationFaculty of Veterinary Medicine. Oslo, Norway.pt_BR
dc.creator.affilliationFederal University of Pará. Laboratory of Wild Animal Biology and Medicine. Castanhal, PA, Brazil.pt_BR
dc.creator.affilliationFederal University of Pará. Laboratory of Wild Animal Biology and Medicine. Castanhal, PA, Brazil / Utrecht University. Faculty of Veterinary Medicine. Utrecht, The Netherlands.pt_BR
dc.identifier.doi10.1071/RD14454-


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