Surveillance of cutaneous leishmaniasis in clinical samples: distribution of Leishmania guyanensis in the state of Amapá, Brazil, 2018
Almeida, Ariely Nunes Ferreira de
Nascimento, Luciana de Cássia Silva do
Sousa, Edith Silvia Moura de Moura
Oliveira, Afonso José Diger de
Sena, Maria Gorete de
Resende, Breno Maués de
Chaves, Raimunda Cleide Gonçalves
Santos, Lourdes Maria Garcez dos
Objective: to investigate Leishmania species in a series of autochthonous cutaneous leishmaniasis (CL) cases in Amapá State, Brazilian Amazon. Methods: this was a descriptive ecological study carried out from January-October/2018 at a reference center for CL diagnosis in Amapá; individuals with CL receiving care from January-May/2018 were recruited; clinical data and skin biopsies were obtained; from extracted DNA (phenol-chloroform) we amplified the hsp70-234 gene region (PCR) for nucleotide sequencing (Applied Biosystems: ABI3500XL). Results: 38 individuals were interviewed, examined and diagnosed; men predominated (28/38; mean age=32.5±11.3); lesions (most ulcers: 37/38) measuring 0,4-10mm (34/38) and ≥11mm (4/38) were multiple in 20/38 individuals; diagnosis of L. braziliensis (1), L. naiffi (1), L. infantum (1), L. (Viannia) sp. (1), L. amazonensis (2) and L. guyanensis (32); individuals infected with L. guyanensis (32/38) lived in 9/10 municipalities represented in the sample, and 17/32 of these had multiple lesions. Conclusion: presence of Leishmania guyanensis predominated and was frequently associated with multiple lesions.
xmlui.dri2xhtml.METS-1.0.item-citationALMEIDA, Ariely Nunes Ferreira de et al. Surveillance of cutaneous leishmaniasis in clinical samples: distribution of Leishmania guyanensis in the state of Amapá, Brazil, 2018. Epidemiologia e Serviços de Saúde, v. 29, n. 1, e2018504, 2020.
xmlui.dri2xhtml.METS-1.0.item-decsPrimaryLeishmaniose Cutânea / patologia
Leishmania guyanensis / parasitologia
Epidemiologia Molecular / métodos