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Environmental enrichment improved learning and memory, increased telencephalic cell proliferation, and induced differential gene expression in Colossoma macropomum

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2020
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Environmental enrichment improved learning and memory, increased telencephalic cell proliferation, and induced differential gene expression in Colossoma macropomum.pdf (6.293xmlui.dri2xhtml.METS-1.0.size-megabytes)
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http://patua.iec.gov.br//handle/iec/4111
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Pereira, Patrick Douglas Corrêa
Henrique, Ediely Pereira
Porfírio, Danillo Monteiro
Crispim, Caio César de Sousa
Campos, Maitê Thaís Barros
Oliveira, Renata Melo de
Silva, Isabella Mesquita Sfair
Guerreiro, Luma Cristina Ferreira
Silva, Tiago Werley Pires da
Silva, Anderson de Jesus Falcão da
Rosa, João Batista da
Azevedo, Dmitre Leonardo Ferreira de
Lima, Cecília Gabriellla Coutinho
Abreu, Cintya Castro de
Santos Filho, Carlos
Diniz, Domingos Luiz Wanderley Picanço
Magalhães, Nara Gyzely de Morais
Diniz, Cristovam Guerreiro
Diniz, Cristovam Wanderley Picanço
Diniz, Daniel Guerreiro
xmlui.dri2xhtml.METS-1.0.item-abstract
Fish use spatial cognition based on allocentric cues to navigate, but little is known about how environmental enrichment (EE) affects learning and memory in correlation with hematological changes or gene expression in the fish brain. Here we investigated these questions in Colossoma macropomum (Teleostei). Fish were housed for 192 days in either EE or in an impoverished environment (IE) aquarium. EE contained toys, natural plants, and a 12-h/day water stream for voluntary exercise, whereas IE had no toys, plants, or water stream. A third plus maze aquarium was used for spatial and object recognition tests. Compared with IE, the EE fish showed greater learning rates, body length, and body weight. After behavioral tests, whole brain tissue was taken, stored in RNA-later, and then homogenized for DNA sequencing after conversion of isolated RNA. To compare read mapping and gene expression profiles across libraries for neurotranscriptome differential expression, we mapped back RNA-seq reads to the C. macropomum de novo assembled transcriptome. The results showed significant differential behavior, cell counts and gene expression in EE and IE individuals. As compared with IE, we found a greater number of cells in the telencephalon of individuals maintained in EE but no significant difference in the tectum opticum, suggesting differential plasticity in these areas. A total of 107,669 transcripts were found that ultimately yielded 64 differentially expressed transcripts between IE and EE brains. Another group of adult fish growing in aquaculture conditions were either subjected to exercise using running water flow or maintained sedentary. Flow cytometry analysis of peripheral blood showed a significantly higher density of lymphocytes, and platelets but no significant differences in erythrocytes and granulocytes. Thus, under the influence of contrasting environments, our findings showed differential changes at the behavioral, cellular, and molecular levels. We propose that the differential expression of selected transcripts, number of telencephalic cell counts, learning and memory performance, and selective hematological cell changes may be part of Teleostei adaptive physiological responses triggered by EE visuospatial and somatomotor stimulation. Our findings suggest abundant differential gene expression changes depending on environment and provide a basis for exploring gene regulation mechanisms under EE in C. macropomum.
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PEREIRA, Patrick Douglas Corrêa et al. Environmental enrichment improved learning and memory, increased telencephalic cell proliferation, and induced differential gene expression in Colossoma macropomum. Frontiers in Pharmacology, v. 11, n. 840, p. 1-14, June 2020.
xmlui.dri2xhtml.METS-1.0.item-decsPrimary
Peixes / anatomia & histologia
Comportamento Animal
Memória Espacial
Aprendizagem Espacial
Enriquecimento Ambiental
Sequenciamento Completo do Genoma / métodos
Cognição
Colossoma macropomum (Teleostei)
Tambaqui
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  • SAHEP - Artigos Científicos

Instituto Evandro Chagas - SVS - MS - 2007-2018 Rodovia BR316 km 7 sn - Levilandia - 67030-000 - Ananindeua - Para - Brasil.
Licença Creative CommonsEste trabalho está licenciado com uma Licença Creative Commons - Atribuição-NãoComercial 4.0 Internacional
Tel: (55 91) 3214-2191
Email: biblioteca@iec.gov.br / clariceneta@iec.gov.br
 

 

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Instituto Evandro Chagas - SVS - MS - 2007-2018 Rodovia BR316 km 7 sn - Levilandia - 67030-000 - Ananindeua - Para - Brasil.
Licença Creative CommonsEste trabalho está licenciado com uma Licença Creative Commons - Atribuição-NãoComercial 4.0 Internacional
Tel: (55 91) 3214-2191
Email: biblioteca@iec.gov.br / clariceneta@iec.gov.br