• Portal do Governo Brasileiro
  • Atualize sua Barra de Governo
Logo
    • English
    • español
    • português (Brasil)
    • English
    • español
    • português (Brasil)
  • xmlui.dri2xhtml.structural.login
xmlui.ArtifactBrowser.ItemViewer.trail 
  •   xmlui.general.dspace_home
  • SEÇÃO DE PARASITOLOGIA
  • SAPAR - Artigos Científicos
  • xmlui.ArtifactBrowser.ItemViewer.trail
  •   xmlui.general.dspace_home
  • SEÇÃO DE PARASITOLOGIA
  • SAPAR - Artigos Científicos
  • xmlui.ArtifactBrowser.ItemViewer.trail
JavaScript is disabled for your browser. Some features of this site may not work without it.

Accuracy of real-time polymerase chain reaction to detect Schistosoma mansoni – infected individuals from an endemic area with low parasite loads

Thumbnail
xmlui.dri2xhtml.METS-1.0.item-date
2020
xmlui.dri2xhtml.METS-1.0.item-files-viewOpen
Accuracy of real-time polymerase chain reaction to detect Schistosoma mansoni – infected individuals from an endemic area with low parasite loads.pdf (538.1xmlui.dri2xhtml.METS-1.0.size-kilobytes)
xmlui.mirage2.itemSummaryView.MetaData
xmlui.ArtifactBrowser.ItemViewer.show_full
xmlui.dri2xhtml.METS-1.0.item-uri
http://patua.iec.gov.br//handle/iec/4140
xmlui.dri2xhtml.METS-1.0.item-author
Magalhães, Fernanda do Carmo
Resende, Samira Diniz
Senra, Carolina
Graeff-Teixeira, Carlos
Enk, Martin Johannes
Coelho, Paulo Marcos Zech
Oliveira, Edward
Corrêa, Deborah Aparecida Negrão
Geiger, Stefan Michael
Carneiro, Mariângela
xmlui.dri2xhtml.METS-1.0.item-abstract
Due to the efforts to control schistosomiasis transmission in tropical countries, a large proportion of individuals from endemic areas present low parasite loads, which hinders diagnosis of intestinal schistosomiasis by the Kato-Katz (KK) method. Therefore, the development of more sensitive diagnostic methods is essential for efficient control measures. The aim was to evaluate the accuracy of a real-time polymerase chain reaction (RT-PCR) to detect Schistosoma mansoni DNA in fecal samples of individuals with low parasite loads. A cross-sectional population-based study was conducted in a rural community (n = 257) in Brazil. POC-CCA® was performed in urine and feces were used for RT-PCR. In addition, fecal exams were completed by 18 KK slides, saline gradient and Helmintex techniques. The combined results of the three parasitological tests detected schistosome eggs in 118 participants (45.9%) and composed the consolidated reference standard (CRS). By RT-PCR, 117 out of 215 tested samples were positive, showing 91.4% sensitivity, 80.2% specificity and good concordance with the CRS (kappa = 0.71). RT-PCR identified 86.9% of the individuals eliminating less than 12 eggs/g of feces, demonstrating much better performance than POC-CCA® (50.8%). Our results showed that RT-PCR is a valuable alternative for the diagnosis of intestinal schistosomiasis in individuals with very low parasite loads.
xmlui.dri2xhtml.METS-1.0.item-citation
MAGALHÃES, Fernanda do Carmo et al. Accuracy of real-time polymerase chain reaction to detect Schistosoma mansoni – infected individuals from an endemic area with low parasite loads. Parasitology, v. 147, n. 10, p. 1140-1148, Sept. 2020.
xmlui.dri2xhtml.METS-1.0.item-decsPrimary
Esquistossomose / parasitologia
Esquistossomose / Diagnóstico
Esquistossomose mansoni / parasitologia
Reação em Cadeia da Polimerase em Tempo Real / métodos
Carga Parasitária
xmlui.dri2xhtml.METS-1.0.item-rights
Acesso Embargado
xmlui.mirage2.itemSummaryView.Collections
  • SAPAR - Artigos Científicos

Instituto Evandro Chagas - SVS - MS - 2007-2018 Rodovia BR316 km 7 sn - Levilandia - 67030-000 - Ananindeua - Para - Brasil.
Licença Creative CommonsEste trabalho está licenciado com uma Licença Creative Commons - Atribuição-NãoComercial 4.0 Internacional
Tel: (55 91) 3214-2191
Email: biblioteca@iec.gov.br / clariceneta@iec.gov.br
 

 

xmlui.ArtifactBrowser.Navigation.head_browse

xmlui.ArtifactBrowser.Navigation.head_all_of_dspacexmlui.ArtifactBrowser.Navigation.communities_and_collectionsxmlui.ArtifactBrowser.Navigation.browse_dateissuedxmlui.ArtifactBrowser.Navigation.browse_authorxmlui.ArtifactBrowser.Navigation.browse_titlexmlui.ArtifactBrowser.Navigation.browse_subjectxmlui.ArtifactBrowser.Navigation.head_this_collectionxmlui.ArtifactBrowser.Navigation.browse_dateissuedxmlui.ArtifactBrowser.Navigation.browse_authorxmlui.ArtifactBrowser.Navigation.browse_titlexmlui.ArtifactBrowser.Navigation.browse_subject

xmlui.EPerson.Navigation.my_account

xmlui.EPerson.Navigation.loginxmlui.EPerson.Navigation.register

Instituto Evandro Chagas - SVS - MS - 2007-2018 Rodovia BR316 km 7 sn - Levilandia - 67030-000 - Ananindeua - Para - Brasil.
Licença Creative CommonsEste trabalho está licenciado com uma Licença Creative Commons - Atribuição-NãoComercial 4.0 Internacional
Tel: (55 91) 3214-2191
Email: biblioteca@iec.gov.br / clariceneta@iec.gov.br