Immune escape mutations in HIV-1 controllers in the Brazilian Amazon region
Gomes, Samara Tatielle Monteiro
Amoras, Ednelza da Silva Graças
Gomes, Érica Ribeiro
Queiroz, Maria Alice Freitas
Sousa Júnior, Edivaldo Costa
Massafra, Janaína Mota de Vasconcelos
Lemos, Poliana da Silva
Vianez Júnior, João Lídio da Silva Gonçalves
Vallinoto, Antonio Carlos Rosário
Background: Human immunodeficiency virus (HIV-1) infection is characterized by high viral replication and a decrease in CD4+ T cells (CD4+TC), resulting in AIDS, which can lead to death. In elite controllers and viremia controllers, viral replication is naturally controlled, with maintenance of CD4+TC levels without the use of antiretroviral therapy (ART). Methods: The aim of the present study was to describe virological and immunological risk factors among HIV-1-infected individuals according to characteristics of progression to AIDS. The sample included 30 treatment-naive patients classified into three groups based on infection duration (> 6 years), CD4+TC count and viral load: (i) 2 elite controllers (ECs), (ii) 7 viremia controllers (VCs) and (iii) 21 nonviremia controllers (NVCs). Nested PCR was employed to amplify the virus genome, which was later sequenced using the Ion PGM platform for subtyping and analysis of immune escape mutations. Results: Viral samples were classified as HIV-1 subtypes B and F. Greater selection pressure on mutations was observed in the group of viremia controllers, with a higher frequency of immunological escape mutations in the genes investigated, including two new mutations in gag. The viral sequences of viremia controllers and nonviremia controllers did not differ significantly regarding the presence of immune escape mutations. Conclusion: The results suggest that progression to AIDS is not dependent on a single variable but rather on a set of characteristics and pressures exerted by virus biology and interactions with immunogenetic host factors.
xmlui.dri2xhtml.METS-1.0.item-citationGOMES, Samara Tatielle Monteiro et al. Immune escape mutations in HIV-1 controllers in the Brazilian Amazon region. BMC Infectious Diseases, v. 20, n. 546, p. 1-11, July 2020.
xmlui.dri2xhtml.METS-1.0.item-decsPrimaryHIV-1 / ultraestrutura
Linfócitos T / imunologia