Evaluation of immunoglobulin M-specific capture enzyme-linked immunosorbent assays and commercial tests for flaviviruses diagnosis by a National Reference Laboratory

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2020xmlui.mirage2.itemSummaryView.MetaData
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http://patua.iec.gov.br//handle/iec/4182xmlui.dri2xhtml.METS-1.0.item-author
Henriques, Daniele Freitas
Nunes, Juliana A. L
Anjos, Maura Viana dos
Melo, Juliana M
Rosário, Wallace O
Azevedo, Raimunda do Socorro da Silva
Chiang, Jannifer Oliveira
Martins, Livia Carício
Santos, Flavia B. dos
Casseb, Livia Medeiros Neves
Vasconcelos, Pedro Fernando da Costa
Rodrigues, Sueli Guerreiro
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Zika and Dengue viruses present considerable immunological cross-reactivity, resulting in a troublesome serodiagnosis due to occurrence of false positive results. Due to Brazil’s wide variety of circulating flaviviruses we aimed to access the use of in house serological tests adapted by National Reference Laboratory for Arboviruses in Brazil and evaluate commercial tests available. We evaluated in house IgM ELISAs for the individual detection of anti-ZIKV, -DENV, and –YFV IgM, against a panel of samples positive for dengue, zika, yellow fever, Rocio, Ilheus, Saint Louis encephalitis, West Nile and chikungunya. We also evaluated two commercial kits for dengue and zika IgM detection recommended by the Brazilian Ministry of Health in 2015. The sensitivity and specificity for the in house ZIKV IgM ELISA was 60.0 % and 88.6 % and for the in house DENV IgM ELISA was 100 % and 82.2 %, respectively. The in house YFV IgM ELISA presented 100 % for both sensitivity and specificity. The Novagnost Zika Virus IgM test presented a sensitivity of 47.3 % and specificity of 85.3 % and the Serion ELISA classic Dengue Virus IgM, 92.8 % and 58.9 %, respectively. Overall, both in house ELISAs for ZIKV and DENV adapted and evaluated here, presented better performances than the commercial kits tested.
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HENRIQUES, Daniele Freitas et al. Evaluation of immunoglobulin M-specific capture enzyme-linked immunosorbent assays and commercial tests for flaviviruses diagnosis by a National Reference Laboratory. Journal of Virological Methods, v. 286, n. 113976, p. xx, Dec. 2020.xmlui.dri2xhtml.METS-1.0.item-decsPrimary
Flavivirus / isolamento & purificaçãoInfecções por Flavivirus
Dengue
Zika Virus
Febre Amarela
Ensaio de Imunoadsorção Enzimática
Imunoglobulina M
Testes Laboratoriais
Vigilância de Produtos Comercializados