The use of the circulating cathodic antigen (Cca) urine cassette assay for the diagnosis and assessment of cure of schistosoma mansoni infections in an endemic
Sousa, Sergei Rodrigo Magalhães de
Nogueira, Joyce Favacho Cardoso
Dias, Isabelle Helena Lima
Fonseca, Álvaro Luan Santana
Geiger, Stefan Michael
Enk, Martin Johannes
INTRODUCTION: Schistosomiasis is a poverty-related disease that affects people in 78 countries worldwide. This study aimed to evaluate the point-of-care circulating cathodic antigen (POC-CCA) test performance using sensitive parasitological methods as a reference standard (RS) in individuals before and after treatment. METHODS: The RS was established by combining the results of 16 Kato-Katz slides and the Helmintex® method. Positivity rates of the POC-CCA test and Kato-Katz and Helmintex® methods were calculated before treatment and 30 days afterward. Furthermore, the sensitivity, specificity, accuracy, and kappa coefficient before treatment were determined by comparing the methods. The cure rate was defined 30 days after treatment. RESULTS: Among the 217 participants, the RS detected a total of 63 (29.0%) positive individuals. The POC-CCA test identified 79 (36.4%) infections. The evaluation of POC-CCA test performance in relation to the RS revealed a sensitivity of 61.9%, specificity of 74.0%, accuracy of 70.5%, and kappa coefficient of 0.33. Out of the 53 remaining participants after treatment, a total of 45 (81.1%) showed egg negative results, and 8 (18.9%) were egg positive according to the RS. A total of 5 (9.4%) egg-positive and 37 (69.8%) egg-negative individuals were positive by the POC-CCA test. CONCLUSIONS: Our data show that the POC-CCA test has potential as an auxiliary tool for the diagnosis of Schistosoma mansoni infection, yielding better results than 16 Kato-Katz slides from three different stool samples. However, the immunochromatographic test lacks sufficient specificity and sensitivity for verifying the cure rate after treatment.
xmlui.dri2xhtml.METS-1.0.item-citationSOUSA, Sergei Rodrigo Magalhães de et al. The use of the circulating cathodic antigen (Cca) urine cassette assay for the diagnosis and assessment of cure of schistosoma mansoni infections in an endemic. Revista da Sociedade Brasileira de Medicina Tropical, v. 53, e20190562, Sept. 2020.
xmlui.dri2xhtml.METS-1.0.item-decsPrimaryEsquistossomose mansoni / parasitologia
Esquistossomose mansoni / diagnóstico
Sensibilidade e Especificidade
Contagem de Ovos de Parasitas