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dc.contributor.authorLobo, Patrícia dos Santos-
dc.contributor.authorCardoso, Jedson Ferreira-
dc.contributor.authorBarata, Rafael Ribeiro-
dc.contributor.authorLemos, Poliana da Silva-
dc.contributor.authorGuerra, Sylvia de Fátima dos Santos-
dc.contributor.authorSoares, Luana da Silva-
dc.contributor.authorNunes, Marcio Roberto Teixeira-
dc.contributor.authorMascarenhas, Joana D’Arc Pereira-
dc.date.accessioned2020-10-26T14:41:35Z-
dc.date.available2020-10-26T14:41:35Z-
dc.date.issued2020-
dc.identifier.citationLOBO, Patrícia dos Santos et al. Near-complete genome of cosavirus A from a child hospitalized with acute gastroenteritis, Brazil. Infection, Genetics and Evolution, v. 85, n. 104555, Nov. 2020.pt_BR
dc.identifier.issn15671348-
dc.identifier.urihttp://patua.iec.gov.br//handle/iec/4190pt_BR
dc.description.abstractAcute gastroenteritis (AG) is responsible for 525,000 deaths worldwide in children under-5-years and is caused by the Human Cosavirus (HCoSV; family Picornaviridae, Genus Cosavirus). Although its health importance, a significant percentage of diarrhea cases (≈ 40 %) still of unknown etiology. In Brazil, few studies have reported HCoSV-A sequences analyzing partial 5' UTR. This study characterized the first near-complete genome of a Cosavirus A (strain AM326) from a child hospitalized with AG in Amazonas state, Northern Brazil. High throughput sequencing (HTS) was performed using the HiSeq™ 2500 platform (Illumina) in one fecal specimen collected from the Surveillance of Rotavirus Network of the Evandro Chagas Institute collected in 2017. Sequence reads were assembled by the De Novo approach using three distinct algorithmic (IDBA-UD, Spades, and MegaHit). The final contig was recovered from the HCoSV-AM326 sample revealing 7,735 nt in length (SRA number SRR12535029; GenBank MT023104) and the genetic characterization, as well as phylogenetic analysis demonstrated a new variant strain from Brazil, highlighting the association of HCoSV-A as a possible causative agent of AG. This finding demonstrates the importance of the metagenomic approach to elucidate cases of diarrhea without a defined etiology, as well as providing a better understanding about the virus genetics, evolution and epidemiology.pt_BR
dc.description.sponsorshipFoundation for Scientific and Technological Development in Health (FIOTEC) Project PRES-012-FIO-16. CNPq grant 304781/2019-3.pt_BR
dc.language.isoengpt_BR
dc.publisherElsevierpt_BR
dc.rightsAcesso Embargadopt_BR
dc.titleNear-complete genome of cosavirus A from a child hospitalized with acute gastroenteritis, Brazilpt_BR
dc.typeArtigopt_BR
dc.subject.decsPrimaryGastroenterite / virologiapt_BR
dc.subject.decsPrimaryGastroenterite / genéticapt_BR
dc.subject.decsPrimaryCosavírus Humano Apt_BR
dc.subject.decsPrimarySequenciamento Completo do Genomapt_BR
dc.subject.decsPrimaryMetagenômicapt_BR
dc.subject.decsPrimaryFilogeniapt_BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Programa de Pós-Graduação em Virologia. Ananindeua, PA, Brasil.pt_BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Programa de Pós-Graduação em Virologia. Ananindeua, PA, Brasil.pt_BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.pt_BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.pt_BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.pt_BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.pt_BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.pt_BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.pt_BR
dc.identifier.doi10.1016/j.meegid.2020.104555-


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