Experimental yellow fever virus infection in the squirrel monkey (Saimiri spp.) I: gross anatomical and histopathological findings in organs at necropsy

xmlui.dri2xhtml.METS-1.0.item-date
2020xmlui.mirage2.itemSummaryView.MetaData
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http://patua.iec.gov.br//handle/iec/4202xmlui.dri2xhtml.METS-1.0.item-author
Ferreira, Milene Silveira
Bezerra Junior, Pedro Soares
Cerqueira, Valíria Duarte
Rivero, Gabriela Riet Correa
Oliveira Junior, Carlos Alberto
Castro, Paulo Henrique Gomes
Silva, Gilmara Abreu da
Silva, Wellington Bandeira da
Imbeloni, Aline Amaral
Sousa, Jorge Rodrigues
Araújo, Ana Paula Sousa
Silva, Franko de Arruda e
Tesh, Robert B
Quaresma, Juarez Antônio Simões
Vasconcelos, Pedro Fernando da Costa
xmlui.dri2xhtml.METS-1.0.item-abstract
BACKGROUND Non-human primates contribute to the spread of the yellow fever virus (YFV) and the establishment of
transmission cycles in endemic areas.
OBJECTIVE To describe the severe histopathological aspects of YFV infection, 10 squirrel monkeys were infected with YFV and
blood, brain, liver, kidney, spleen, heart, lung, lymph node and stomach were collected at 1-7, 10, 20 and 30 days post-infection (dpi).
METHODS Histopathological analysis and detection of the genome and viral antigens and neutralising antibodies were performed
by RT-PCR, immunohistochemistry and neutralisation test, respectively.
FINDINGS Only one animal died from the experimental infection. The genome and viral antigens were detected in all investigated
organs (1-30 dpi) and the neutralising antibodies from seven to 30 dpi. The brain contained perivascular haemorrhage (6 dpi);
in the liver, midzonal haemorrhage and lytic necrosis (6 dpi) were observed. The kidney had bleeding in the Bowman’s capsule
and tubular necrosis (6 dpi). Pyknotic lymphocytes were observed in the spleen (1-20 dpi), the lung had haemorrhage (2-6 dpi),
in the endocardium it contained nuclear pyknosis and necrosis (2-3 dpi) and the stomach contained blood in the lumen (6 dpi).
MAIN FINDINGS Squirrel monkeys reliably reproduced the responses observed in human cases of yellow fever and, therefore,
constitute an excellent experimental model for studies on the pathophysiology of the disease.
xmlui.dri2xhtml.METS-1.0.item-citation
FERREIRA, Milene Silveira et al. Experimental yellow fever virus infection in the squirrel monkey (Saimiri spp.) I: gross anatomical and histopathological findings in organs at necropsy. Memórias do Instituto Oswaldo Cruz, v. 115, e190501, Nov. 2020.xmlui.dri2xhtml.METS-1.0.item-decsPrimary
Febre Amarela / veterináriaVírus da Febre Amarela / patogenicidade
Saimiri / anatomia & histologia