Further insights into the eco-epidemiology of American cutaneous leishmaniasis in the Belem metropolitan region, Pará State, Brazil
Gonçalves, Lucas Pantoja
Santos, Thiago Vasconcelos dos
Campos, Marliane Batista
Lima, Luciana Vieira do Rêgo
Ishikawa, Edna Aoba Yassui
Silveira, Fernando Tobias
Ramos, Patrícia Karla Santos
Introduction: In the Belém Metropolitan Region (BMR), Pará State, Brazil, American cutaneous leishmaniasis (ACL) is endemic; however, very little is known regarding its causative agents. Therefore, we used our standard diagnostic approach combined with an RNA polymerase II largest subunit (RNAPOIILS)-polymerase chain reaction (PCR) followed by analysis of restriction fragment length polymorphism (PCR-RFLP) to identify Leishmania spp. ACL agents in this region. Methods: Thirty-two Leishmania spp. isolates from patients with ACL in the BMR during 1995-2018 were analyzed. Leishmania spp. DNA samples were amplified using the primers RPOR2/RPOF2, and the 615-bp PCR products were subjected to enzymatic digestion using TspRI and HgaI endonucleases. Results: ACL etiological agents in the BMR comprised Leishmania (Viannia) lindenbergi (43.7%) followed by Leishmania (Viannia) lainsoni (34.4%), Leishmania (Leishmania) amazonensis (12.5%), and Leishmania (Viannia) braziliensis (9.4%). Conclusions: To our knowledge, the results of the study revealed for the first time that L. (V.) lindenbergi and L. (V.) lainsoni are the main ACL agents in BMR.
xmlui.dri2xhtml.METS-1.0.item-citationGONÇALVES, Lucas Pantoja et al. Further insights into the eco-epidemiology of American cutaneous leishmaniasis in the Belem metropolitan region, Pará State, Brazil. Revista da Sociedade Brasileira de Medicina Tropical, v. 53, e20200255, p. 1-8, Dec. 2020.
xmlui.dri2xhtml.METS-1.0.item-decsPrimaryLeishmaniose Cutânea / patologia
Leishmaniose Cutânea / etiologia
Reação em Cadeia da Polimerase / métodos