Further insights into the eco-epidemiology of American cutaneous leishmaniasis in the Belem metropolitan region, Pará State, Brazil

xmlui.dri2xhtml.METS-1.0.item-date
2020xmlui.mirage2.itemSummaryView.MetaData
xmlui.ArtifactBrowser.ItemViewer.show_fullxmlui.dri2xhtml.METS-1.0.item-uri
http://patua.iec.gov.br//handle/iec/4222xmlui.dri2xhtml.METS-1.0.item-author
Gonçalves, Lucas Pantoja
Santos, Thiago Vasconcelos dos
Campos, Marliane Batista
Lima, Luciana Vieira do Rêgo
Ishikawa, Edna Aoba Yassui
Silveira, Fernando Tobias
Ramos, Patrícia Karla Santos
xmlui.dri2xhtml.METS-1.0.item-abstract
Introduction: In the Belém Metropolitan Region (BMR), Pará State, Brazil, American cutaneous leishmaniasis (ACL) is endemic; however,
very little is known regarding its causative agents. Therefore, we used our standard diagnostic approach combined with an RNA polymerase
II largest subunit (RNAPOIILS)-polymerase chain reaction (PCR) followed by analysis of restriction fragment length polymorphism
(PCR-RFLP) to identify Leishmania spp. ACL agents in this region. Methods: Thirty-two Leishmania spp. isolates from patients with ACL
in the BMR during 1995-2018 were analyzed. Leishmania spp. DNA samples were amplified using the primers RPOR2/RPOF2, and the
615-bp PCR products were subjected to enzymatic digestion using TspRI and HgaI endonucleases. Results: ACL etiological agents in the
BMR comprised Leishmania (Viannia) lindenbergi (43.7%) followed by Leishmania (Viannia) lainsoni (34.4%), Leishmania (Leishmania)
amazonensis (12.5%), and Leishmania (Viannia) braziliensis (9.4%). Conclusions: To our knowledge, the results of the study revealed for
the first time that L. (V.) lindenbergi and L. (V.) lainsoni are the main ACL agents in BMR.
xmlui.dri2xhtml.METS-1.0.item-citation
GONÇALVES, Lucas Pantoja et al. Further insights into the eco-epidemiology of American cutaneous leishmaniasis in the Belem metropolitan region, Pará State, Brazil. Revista da Sociedade Brasileira de Medicina Tropical, v. 53, e20200255, p. 1-8, Dec. 2020.xmlui.dri2xhtml.METS-1.0.item-decsPrimary
Leishmaniose Cutânea / patologiaLeishmaniose Cutânea / etiologia
Reação em Cadeia da Polimerase / métodos