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Urine qPCR diagnoses over the entire clinical-immunological spectrum of human Leishmania (L.) infantum chagasi-infections in the Brazilian Amazon

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2021
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Urine qPCR diagnoses over the entire clinical-immunological spectrum of human Leishmania (L.) infantum chagasi-infections in the Brazilian Amazon.pdf (538.1xmlui.dri2xhtml.METS-1.0.size-kilobytes)
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http://patua.iec.gov.br//handle/iec/4237
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Ramos, Patrícia Karla Santos
Gonçalves, Lucas Pantoja
Alves, Ana Camila Oliveira
Casseb, Samir Mansour Moraes
Lima, Luciana Vieira do Rêgo
Campos, Marliane Batista
Santos, Thiago Vasconcelos dos
Silveira, Fernando Tobias
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The clinical-immunological spectrum of human Leishmania (L.) infantum chagasi-infections in the Brazilian Amazon has been defined using DTH/IFAT-IgG immune assays and the clinical statuses of infected individuals, revealing five profiles: three asymptomatic [Asymptomatic Infection (AI), Subclinical Resistant Infection (SRI), and Indeterminate Initial Infection (III)], and two symptomatic profiles [Subclinical Oligosymptomatic Infection (SOI) and Symptomatic Infection (SI = American visceral leishmaniasis/AVL)]. We evaluated the diagnostic potential of urine qPCR over the entire spectrum of infection. Resine Instagene Matrix® was used for DNA extraction from urinary sediment, with amplification carried out using SYBR® Green Taq with the RV1 and RV2 primers. We examined urine samples from 151 individuals from an endemic area of AVL in Pará State in the Brazilian Amazon, including: 91 (60.3%) with diagnoses of previous infections [13 (14.3%) sharing the AI profile, 13 (14.3%) with the SRI profile, 43 (47.2%) with III, 12 (13.2%) with SI (treated AVL), and 10 (11%) with SI (untreated AVL)]; sixty (39.7%) were DTH(-)/IFAT-IgG(-) (the uninfected group). The urine qPCR was positive in 61.5% of both the AI and SRI profiles, 65% of the III profile, 50% of treated AVL, 100% of untreated AVL, and 6.7% of the uninfected group. Those results confirmed the urine qPCR diagnosis in 100% of untreated AVL cases as well as in more than 60% of the cases with asymptomatic AI, SRI, and III profiles - indicating it as a promising tool for monitoring the evolution of human L. (L.) infantum chagasi-infections in endemic areas.
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RAMOS, Patrícia Karla Santos et al. Urine qPCR diagnoses over the entire clinical-immunological spectrum of human Leishmania (L.) infantum chagasi-infections in the Brazilian Amazon. Parasitology International, v. 81, n. xx, p. xx, Apr. 2021.
xmlui.dri2xhtml.METS-1.0.item-decsPrimary
Leishmania infantum / isolamento & purificação
Urina / parasitologia
Reação em Cadeia da Polimerase / Métodos
qPCR
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Instituto Evandro Chagas - SVS - MS - 2007-2018 Rodovia BR316 km 7 sn - Levilandia - 67030-000 - Ananindeua - Para - Brasil.
Licença Creative CommonsEste trabalho está licenciado com uma Licença Creative Commons - Atribuição-NãoComercial 4.0 Internacional
Tel: (55 91) 3214-2191
Email: biblioteca@iec.gov.br / clariceneta@iec.gov.br
Suporte ao site - Wesin Alves
 

 

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Instituto Evandro Chagas - SVS - MS - 2007-2018 Rodovia BR316 km 7 sn - Levilandia - 67030-000 - Ananindeua - Para - Brasil.
Licença Creative CommonsEste trabalho está licenciado com uma Licença Creative Commons - Atribuição-NãoComercial 4.0 Internacional
Tel: (55 91) 3214-2191
Email: biblioteca@iec.gov.br / clariceneta@iec.gov.br
Suporte ao site - Wesin Alves