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Genomic and transcriptomic characterization of the human glioblastoma cell line AHOL1

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2021
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Genomic and transcriptomic characterization of the human glioblastoma cell line AHOL1.pdf (800.4Kb)
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URI
http://patua.iec.gov.br//handle/iec/4244
Autor
Ferreira, W. A. S
Amorim, C. K. N
Burbano, R. R
Villacis, R. A. R
Marchi, F. A
Medina, T. S
Lima, M. M. C. de
Oliveira, Edivaldo Herculano Corrêa de
Resumo
Cancer cell lines are widely used as in vitro models of tumorigenesis, facilitating fundamental discoveries in cancer biology and translational medicine. Currently, there are few options for glioblastoma (GBM) treatment and limited in vitro models with accurate genomic and transcriptomic characterization. Here, a detailed characterization of a new GBM cell line, namely AHOL1, was conducted in order to fully characterize its molecular composition based on its karyotype, copy number alteration (CNA), and transcriptome profiling, followed by the validation of key elements associated with GBM tumorigenesis. Large numbers of CNAs and differentially expressed genes (DEGs) were identified. CNAs were distributed throughout the genome, including gains at Xq11.1-q28, Xp22.33-p11.1, Xq21.1-q21.33, 4p15.1-p14, 8q23.2-q23.3 and losses at Yq11.21-q12, Yp11.31-p11.2, and 15q11.1-q11.2 positions. Nine druggable genes were identified, including HCRTR2, ETV1, PTPRD, PRKX, STS, RPS6KA6, ZFY, USP9Y, and KDM5D. By integrating DEGs and CNAs, we identified 57 overlapping genes enriched in fourteen pathways. Altered expression of several cancer-related candidates found in the DEGs-CNA dataset was confirmed by RT-qPCR. Taken together, this first comprehensive genomic and transcriptomic landscape of AHOL1 provides unique resources for further studies and identifies several druggable targets that may be useful for therapeutics and biologic and molecular investigation of GBM.
Referência
FERREIRA, W. A. S. et al. Genomic and transcriptomic characterization of the human glioblastoma cell line AHOL1. Brazilian Journal of Medical and Biological Research, v. 54, n. 3, p. 1-12, Jan. 2021.
DeCs
Neoplasias Encefálicas
Glioblastoma / genética
Glioma
Hibridização Genômica Comparativa / métodos
Direito Autoral
Acesso Aberto
Coleções
  • SAMAM - Artigos Científicos

Instituto Evandro Chagas - SVS - MS - 2007-2018 Rodovia BR316 km 7 sn - Levilandia - 67030-000 - Ananindeua - Para - Brasil.
Licença Creative CommonsEste trabalho está licenciado com uma Licença Creative Commons - Atribuição-NãoComercial 4.0 Internacional
Tel: (55 91) 3214-2191
Email: biblioteca@iec.gov.br / clariceneta@iec.gov.br
 

 

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Instituto Evandro Chagas - SVS - MS - 2007-2018 Rodovia BR316 km 7 sn - Levilandia - 67030-000 - Ananindeua - Para - Brasil.
Licença Creative CommonsEste trabalho está licenciado com uma Licença Creative Commons - Atribuição-NãoComercial 4.0 Internacional
Tel: (55 91) 3214-2191
Email: biblioteca@iec.gov.br / clariceneta@iec.gov.br