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dc.contributor.authorFerreira, W. A. S-
dc.contributor.authorAmorim, C. K. N-
dc.contributor.authorBurbano, R. R-
dc.contributor.authorVillacis, R. A. R-
dc.contributor.authorMarchi, F. A-
dc.contributor.authorMedina, T. S-
dc.contributor.authorLima, M. M. C. de-
dc.contributor.authorOliveira, Edivaldo Herculano Corrêa de-
dc.date.accessioned2021-02-03T23:22:18Z-
dc.date.available2021-02-03T23:22:18Z-
dc.date.issued2021-
dc.identifier.citationFERREIRA, W. A. S. et al. Genomic and transcriptomic characterization of the human glioblastoma cell line AHOL1. Brazilian Journal of Medical and Biological Research, v. 54, n. 3, p. 1-12, Jan. 2021.pt_BR
dc.identifier.issn1414-431X-
dc.identifier.urihttp://patua.iec.gov.br//handle/iec/4244-
dc.description.abstractCancer cell lines are widely used as in vitro models of tumorigenesis, facilitating fundamental discoveries in cancer biology and translational medicine. Currently, there are few options for glioblastoma (GBM) treatment and limited in vitro models with accurate genomic and transcriptomic characterization. Here, a detailed characterization of a new GBM cell line, namely AHOL1, was conducted in order to fully characterize its molecular composition based on its karyotype, copy number alteration (CNA), and transcriptome profiling, followed by the validation of key elements associated with GBM tumorigenesis. Large numbers of CNAs and differentially expressed genes (DEGs) were identified. CNAs were distributed throughout the genome, including gains at Xq11.1-q28, Xp22.33-p11.1, Xq21.1-q21.33, 4p15.1-p14, 8q23.2-q23.3 and losses at Yq11.21-q12, Yp11.31-p11.2, and 15q11.1-q11.2 positions. Nine druggable genes were identified, including HCRTR2, ETV1, PTPRD, PRKX, STS, RPS6KA6, ZFY, USP9Y, and KDM5D. By integrating DEGs and CNAs, we identified 57 overlapping genes enriched in fourteen pathways. Altered expression of several cancer-related candidates found in the DEGs-CNA dataset was confirmed by RT-qPCR. Taken together, this first comprehensive genomic and transcriptomic landscape of AHOL1 provides unique resources for further studies and identifies several druggable targets that may be useful for therapeutics and biologic and molecular investigation of GBM.pt_BR
dc.description.sponsorshipThis research was funded by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (Grant Number: 455078/2014-9) and Evandro Chagas Institute.pt_BR
dc.language.isoengpt_BR
dc.publisherAssociação Brasileira de Divulgação Científicapt_BR
dc.rightsAcesso Abertopt_BR
dc.titleGenomic and transcriptomic characterization of the human glioblastoma cell line AHOL1pt_BR
dc.typeArtigopt_BR
dc.subject.decsPrimaryNeoplasias Encefálicaspt_BR
dc.subject.decsPrimaryGlioblastoma / genéticapt_BR
dc.subject.decsPrimaryGliomapt_BR
dc.subject.decsPrimaryHibridização Genômica Comparativa / métodospt-BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Cultura de Tecidos e Citogenética. Ananindeua, PA, Brasilpt_BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Cultura de Tecidos e Citogenética. Ananindeua, PA, Brasilpt_BR
dc.creator.affilliationUniversidade Federal do Pará. Instituto de Ciências Biológicas. Laboratório de Citogenética Humana. Belém, PA, Brasil / Hospital Universitário João de Barros Barreto. Núcleo de Pesquisas em Oncologia. Belém, PA, Brasil / Hospital Ophir Loyola. Laboratório de Biologia Molecular. Belém, PA, Brasil.pt_BR
dc.creator.affilliationUniversidade de Brasília. Instituto de Ciências Biológicas. Departamento de Genética e Morfologia. Brasília, DF, Brasil.pt_BR
dc.creator.affilliationA.C. Camargo Cancer Center. Centro Internacional de Pesquisa. São Paulo, SP, Brasil.pt_BR
dc.creator.affilliationUniversidade Federal do Pará. Instituto de Ciências Biológicas. Faculdade de Biomedicina. Belém, PA, Brasil.pt_BR
dc.creator.affilliationMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Cultura de Tecidos e Citogenética. Ananindeua, PA, Brasil / Universidade Federal do Pará. Instituto de Ciências Exatas e Naturais. Faculdade de Ciências Naturais. Belém, PA, Brasil.pt_BR
dc.identifier.doi/10.1590/1414-431x20209571-


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