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Metabolic and oxidative impairments in human salivary gland cells line exposed to MeHg

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2021
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Metabolic and oxidative impairments in human salivary gland cells line exposed to MeHg.pdf (538.1xmlui.dri2xhtml.METS-1.0.size-kilobytes)
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http://patua.iec.gov.br//handle/iec/4274
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Nogueira, Lygia Sega
Vasconcelos, Carolina P
Mitre, Geovanni Pereira
Kataoka, Maria Sueli da Silva
Bittencourt, Leonardo Oliveira
Lima, Marcelo de Oliveira
Oliveira, Edivaldo Herculano Corrêa de
Crespo-López, Maria Elena
Lima, Rafael Rodrigues
xmlui.dri2xhtml.METS-1.0.item-abstract
Background/aim: The ingestion of contaminated seafood by MeHg is considered the main route of human exposure, turning the salivary gland one important target organ. The salivary glands play critical roles in maintaining oral health homeostasis, producing saliva that maintains the oral microbiota, initiation of the digestion of macromolecules, and being essential in maintaining the integrity of the adjacent soft tissues and teeth. Thus, this study aimed to investigate the effects of MeHg exposure on human salivary gland cells line. Methods: Cells were exposed to 1–6 μM of MeHg for 24 h, and analysis of toxicity was performed. Based on these results, the LC50 was calculated and two concentrations were chosen (0.25 and 2.5 μM MeHg) to evaluate intracellular mercury (Hg) accumulation (THg), metabolic viability and oxidative stress parameters (GSH:GSSG ratio, lipid peroxidation, protein oxidation and DNA damage). Results: The results demonstrated accumulation of THg as we increased the MeHg concentrations in the exposure and, the higher the dose, the lower is the cell metabolic response. In addition, the 2.5 μM MeHg concentration also triggered oxidative stress in human salivary gland cells by depleting the antioxidant competence of GSH:GSSG ratio and increasing lipid peroxidation and proteins carbonyl levels, but no damages to DNA integrity. Conclusion: In conclusion, although these two elected doses did not show lethal effects, the highest dose triggered oxidative stress and new questionings about long-term exposure models are raised to investigate furthers cellular damages to human salivary gland cells caused by MeHg exposure to extrapolate in a translational perspective.
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NOGUEIRA, Lygia Sega et al. Metabolic and oxidative impairments in human salivary gland cells line exposed to MeHg. Journal of Trace Elements in Medicine and Biology, v. 66, n. 126747, July 2021.
xmlui.dri2xhtml.METS-1.0.item-decsPrimary
Glândulas Salivares / patologia
Saúde Bucal
Mercúrio / toxicidade
Estresse Oxidativo
Produtos da Oxidação Avançada de Proteínas
Peroxidação de Lipídeos
Alimentos Marinhos
Microbiota
Ingestão de Alimentos
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  • SAMAM - Artigos Científicos

Instituto Evandro Chagas - SVS - MS - 2007-2018 Rodovia BR316 km 7 sn - Levilandia - 67030-000 - Ananindeua - Para - Brasil.
Licença Creative CommonsEste trabalho está licenciado com uma Licença Creative Commons - Atribuição-NãoComercial 4.0 Internacional
Tel: (55 91) 3214-2191
Email: biblioteca@iec.gov.br / clariceneta@iec.gov.br
 

 

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Instituto Evandro Chagas - SVS - MS - 2007-2018 Rodovia BR316 km 7 sn - Levilandia - 67030-000 - Ananindeua - Para - Brasil.
Licença Creative CommonsEste trabalho está licenciado com uma Licença Creative Commons - Atribuição-NãoComercial 4.0 Internacional
Tel: (55 91) 3214-2191
Email: biblioteca@iec.gov.br / clariceneta@iec.gov.br