The crab heparin-like compound exhibits a strong inhibitory effect on infections by dengue virus-2
Almeida, Marlyanne M. C. S. de
A. Júnior, Renato F. de
Aquino, Ana A. de
Pinto, Lívia de Lourdes
Machado, Paula R. L
Chavante, Suely F
Farias, Kleber Juvenal Silva
Andrade, Giulianna P. V. de
Background: According to the World Health Organization (WHO), two-fifths of the world population is at risk of infection by DENV. There are no safe and effective vaccines estab-lished. Sulfated glycosaminoglycans such as heparin, used as anticoagulants, inhibit the initial step of dengue viral replication. Recently, an isolated heparin analogue Goniopsis cruentata (cCTH) has presented a low anticoagulant effect with reduced bleeding risk. Methods: The antiviral activity of cCTH and heparin compounds against DENV-2 in Vero cell culture was determined by quantitative RT-PCR (qRT-PCR) and titration. For this, four trials were carried out: treatment of the cells for 2 h before viral inoculation, concomitant viral inoculation treatment, treatment after viral inoculation and virucidal assay. Subsequently, the culture superna-tants were collected for periods of 24, 48 and 72 h. Results: Our results demonstrated that cCTH and heparin showed antiviral activity against DENV-2. Conclusion: These data suggest that both compounds prevented viral replication in cultured Vero cells.
xmlui.dri2xhtml.METS-1.0.item-citationALMEIDA, Marlyanne M. C. S. de et al. The crab heparin-like compound exhibits a strong inhibitory effect on infections by dengue virus-2. Anti-Infective Agents, v. 19, n. 1, p. 12-18, 2021.
xmlui.dri2xhtml.METS-1.0.item-decsPrimaryDengue / virologia
Heparina / análise
Células Vero / citologia
Reação em Cadeia da Polimerase em Tempo Real / métodos