HEV infection in swine from Eastern Brazilian Amazon: evidence of co-infection by different subtypes
Souza, Alex Junior Souza de
Gouvêa, Michele Soares Gomes
Soares, Manoel do Carmo Pereira
Pinho, João Renato Rebello
Malheiros, Andreza Pinheiro
Carneiro, Liliane Almeida
Santos, Debora Regina Lopes dos
Pereira, Washington Luiz Assunção
Hepatitis E virus (HEV) is a fecal-orally transmitted member of the genus Hepevirus that causes acute hepatitis in humans and is widely distributed throughout the world. Pigs have been reported as the main source of genotypes 3 and 4 infection to humans in nonendemic areas. To investigate HEV infection in pigs from different regions of Pará state (Eastern Brazilian Amazon), we performed serological and molecular analyses of serum, fecal and liver samples from 151 adult pigs slaughtered between April and October 2010 in slaughterhouses in the metropolitan region of Belém, Pará. Among the animals tested, 8.6 per cente(13/151) were positive for anti-HEV IgG but not for anti-HEV IgM. HEV RNA was detected in 4.8 per cent (22/453) of the samples analyzed and 9.9 per cent (15/151) of the animals had at least one positive sample. Phylogenetic analysis showed that all sequences belonged to genotype 3 that were related to human isolates from other non-endemic regions, suggesting that theisolates had zoonotic potential. Subtypes 3c and 3f were simultaneously detected in some pigs, suggesting co-infection by more than one strain and/or the presence of a recombinant virus. These results constitute the first molecular and serologic evidence of swine HEVcirculation in the Eastern Brazilian Amazon.
xmlui.dri2xhtml.METS-1.0.item-citationSOUZA, Alex Junior Souza de et al. HEV infection in swine from Eastern Brazilian Amazon: evidence of co-infection by different subtypes. Comparative Immunology, Microbiology and Infectious Diseases, v. 35, n. 5, p. 477-485, 2012.
xmlui.dri2xhtml.METS-1.0.item-decsPrimaryVírus da Hepatite / isolamento & purificação
Vírus da Hepatite E
Região Amazônica (BR)