Cytogenetic, growth rate and virus susceptibility differences in sublines of H. Ep. 2 cells
Autor
Mallmann, M. Clara
Pinheiro Filho, Francisco de Paula
Salzano, F. M
Ayres, M
Resumo
Two sublines of H. Ep. 2, obtained from New Haven (NH) and São Paulo
(SP) respectively, were studied in relation to their morphology, growth rate,
virus susceptibility and chromosomes. The São Paulo subline was divided
into two, one cultivated in Hanks lactalbumin medium plus 20% of calf
serum and the other in modified Eagle's medium, to investigate the possible
influence of the medium of cultivation on these variables. This influence
proved to be small. But significant differences were observed between the
two sublines, H. Ep. 2 NH showing a lowered rate of growth, less susceptibility
to virus infections and a higher modal chromosome number. The
changes in chromosome number were of such a nature that there was practically
no overlapping in the range of variation in the two sublines. Groups
B, D and F were the most affected.
Referência
MALLMANN, M. Clara et al. Cytogenetic, growth rate and virus susceptibility differences in sublines of H. Ep. 2 cells. Cytologia, v. 35, n. 2, p. 262-270, 1970.DeCs
CarcinomaDermatomicoses / imunologia
Modelos Animais de Doenças
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